The 1964 Titleholders Championship was the 25th Titleholders Championship, held April 23–26 at Augusta Country Club in Augusta, Georgia. Reigning champion Marilynn Smith successfully defended her title, one stroke ahead of runner-up Mickey Wright. The two had met in an 18-hole playoff the previous year which was decided on the final green. Wright had won the title in 1961 and 1962.

It was the second and final major title for Smith. Her second round 66 (−6) on Friday set several records for this championship, including lowest round, lowest score after 36 holes at 139 (−5), 54 holes at 216 (even), and 72 holes at 289 (+1). Her Friday inward nine score of 31 was also a record, by two strokes.

In the third round on Saturday, scores soared in the rain and cold wind. Smith's 77 (+5) kept the lead at an even-par 216 for 54 holes, three shots ahead of Wright.

Sunday, April 26, 1964

Place	Player	Score	To par	Money ($)
1	Marilynn Smith	73-66-77-73=289	+1	1,300
2	Mickey Wright	74-70-75-71=290	+2	1,000
3	Betsy Rawls	71-77-74-72=294	+6	800
T4	Ruth Jessen	77-72-74-73=296	+8	625
	Judy Kimball	71-72-78-75=296
T6	Clifford Ann Creed	75-69-79-74=297	+9	500
	Shirley Englehorn	75-75-73-74=297
8	Mary Mills	74-74-78-72=298	+10	400
9	Louise Suggs	73-73-76-77=299	+11	350
T10	Marlene Hagge	75-72-78-75=300	+12	275
	Sandra Haynie	74-75-76-75=300
	Judy Rankin	75-73-76-76=300

Source:

33°28′59″N 82°00′40″W / 33.483°N 82.011°W / 33.483; -82.011