Acholi Inn is a hotel in Gulu, the largest city in Northern Uganda.

The hotel is located in the heart of Gulu, at 6/4 Elizabeth Road, approximately 2 kilometres (1.2 mi), north of the central business district of the town. The coordinates of Acholi Inn are:2°46'53.0"N, 32°17'52.0"E (Latitude:2.781389; Longitude:32.297778).

The hotel was built during Uganda's colonial era and was commissioned by the British in 1936. At Independence in 1962, it was one of about a dozen hotels that constituted Uganda Hotels Limited, a government parastatal during the Obote I regime. In 1995, the hotel was privatized and has undergone refurbishment.

As of July 2014, Acholi Inn is privately owned, and detailed shareholding in the establishment is not publicly known.

Acholi Inn has 120 regular rooms, 12 VIP suites, a swimming pool, a health club, a gymnasium, a sauna, a steam bath, and a massage parlour. Its main conference room can accommodate up to 1,000 guests. It also has an Internet café, a restaurant, a cocktail bar, satellite television and air conditioning.

02°46′53″N 32°17′52″E / 2.78139°N 32.29778°E / 2.78139; 32.29778