Highway 28 is a 293-kilometre (182 mi) highway in north-central Alberta, Canada that connects Edmonton to Cold Lake. It begins at Yellowhead Trail (Highway 16) in Edmonton as 97 Street NW, running through the city's north suburbs before entering Sturgeon County and passing CFB Edmonton. After merging with Highway 28A near Gibbons it winds through agricultural lands of north-central Alberta, roughly paralleling the North Saskatchewan River until Smoky Lake before continuing east through St. Paul County to Bonnyville. It turns northeast to CFB Cold Lake, before ending at Lakeshore Drive in the city of Cold Lake shortly thereafter.

The highway is a component of Canada's National Highway System. Between Highway 28A near Gibbons and the intersection with Highway 63 near Radway, it forms part of the Edmonton-Fort McMurray corridor and is designated as a core route. For the remainder of the route from Radway to the eastern end at Cold Lake, it is designated as a feeder route.

Highway 28 was built in 1961, connecting Alberta's Lakeland to Edmonton by gravel road for the first time. Construction of the highway required splitting Mann Lake in two, creating Upper and Lower Mann Lake.

Prior to 2006, Highway 28 ran through St. Paul. A 46 km (29 mi) section of the current highway between Ashmont and Hoselaw was formerly designated as Highway 28A, a bypass of St. Paul. As part of an effort to simplify highway route numbering in the region, this section was re-signed as Highway 28 in 2006 forming a more contiguous route between Edmonton and Cold Lake, while Highway 28 through St. Paul was re-signed as Highway 29.

Highway 28X was a 14-kilometre (9 mi) spur route of Highway 28. It began at Highway 28, approximately 10 kilometres (6 mi) south of Cold Lake, and travelled to the Saskatchewan boundary where it continued east as Saskatchewan Highway 55. In c. 1977, Highway 28X was part of a number of highways which were renumbered when Alberta Highway 55 was established between Athabasca and the Saskatchewan border.

Alberta Transportation ultimately intends to upgrade the entire Edmonton-Fort McMurray corridor to a divided highway, which would include twinning of Highway 28 from Highway 28A to Highway 63.

Starting from the west end of Highway 28:

Rural/specialized municipality	Location	km	mi	Destinations	Notes
City of Edmonton		−4.4	−2.7	Jasper Avenue	Former Hwy 28 southern terminus
		0.0	0.0	Yellowhead Trail (Highway 16 (TCH/YH)) – Lloydminster, Jasper	Single-point urban interchange; Hwy 16; exit 389; Hwy 28 travels north
		7.3	4.5	Anthony Henday Drive (Highway 216)	Interchange; Hwy 216 exit 39
Sturgeon County	​	8.1	5.0	Former Highway 28A north	Interchange permanently closed and removed
		12.0	7.5	Sturgeon Road	Access to CFB Edmonton
	Namao	15.3	9.5	Highway 37 – Onoway, Fort Saskatchewan
	​	25.1	15.6	Highway 642 west – Morinville
		27.7	17.2	Highway 803 north	Hwy 28 turns east; directional signage changes
	Bon Accord	33.0	20.5
	Gibbons	39.9	24.8	Highway 28A south – Edmonton	Trumpet interchange
	​	53.3	33.1	Highway 651 west – Legal
	Redwater	59.9	37.2	Highway 38 east (48 Avenue) – Bruderheim, Two Hills
Thorhild County	​	69.9	43.4	Highway 827 north – Egremont, Thorhild, Athabasca
		76.4	47.5	Highway 63 north – Lac La Biche, Fort McMurray Highway 829 south – Redwater
	Radway	82.6	51.3	UAR 158 south
Smoky Lake County	​	93.0	57.8	Highway 831 – Boyle, Lamont
	Waskatenau	94.1	58.5	UAR 74 south
	Warspite	104.4	64.9	UAR 104 south
	Smoky Lake	115.7	71.9	Highway 855 – Caslan, Andrew, Mundare
		116.5	72.4	UAR 156 south
	​	139.0	86.4	Highway 857 south – Willingdon, Vegreville UAR 217 north – Bellis
		145.5	90.4	Highway 36 north – Lac La Biche	West end of Hwy 36 concurrency
	Vilna	153.0	95.1	UAR 116 south
	​	154.3	95.9	Highway 859 south
	Spedden	166.5	103.5	PAR 120 north – Garner Lake Provincial Park
County of St. Paul No. 19	​	169.7	105.4	Highway 866 north
	Ashmont	176.7	109.8	Highway 36 south – St. Paul, Two Hills	East end of Hwy 36 concurrency
	​	185.1	115.0	Highway 867 north
		198.4	123.3	Highway 881 – Therien, St. Vincent, St. Paul
M.D. of Bonnyville No. 87	​	206.5	128.3	Highway 882 north – Glendon
	Hoselaw	223.1	138.6	Highway 41 south – St. Paul, Elk Point, Vermilion	West end of Hwy 41 concurrency
	​	233.3	145.0	UAR 96 west – Bonnyville Beach
	Bonnyville	240.6	149.5	Highway 41 north (55 Street) – La Corey	East end of Hwy 41 concurrency
		242.5	150.7	Highway 659 east (50 Avenue) – Lloydminster
	​	247.2	153.6	Highway 660 west
	Ardmore	260.6	161.9	Highway 892
	Beaver Crossing	278.6	173.1	Crosses the Beaver River
	​	280.4	174.2	Highway 55 east – Pierceland, Meadow Lake, Prince Albert To Highway 897 south – Elizabeth Metis Settlement, Marwayne, Lloydminster	South end of Hwy 55 wrong-way concurrency
City of Cold Lake		285.6	177.5	50 Avenue / Centre Avenue – CFB Cold Lake	Former Hwy 897
		290.6	180.6	Highway 55 west / 16 Avenue – Cold Lake Provincial Park, Lac La Biche	North end of Hwy 55 wrong-way concurrency
		293.0	182.1	Lakeshore Drive
1.000 mi = 1.609 km; 1.000 km = 0.621 mi       Closed/former       Concurrency terminus