Amisfield railway station was a station which served Amisfield, in the Scottish county of Dumfries and Galloway. It was served by trains on a local line which ran between the Caledonian Main Line (now known as the West Coast Main Line) at Lockerbie and the Castle Douglas and Dumfries Railway at Dumfries. The latter station is now the nearest to Amisfield.

Opened by the Dumfries, Lochmaben and Lockerbie Railway, then part of the Caledonian Railway it became part of the London Midland and Scottish Railway during the Grouping of 1923, passing on to the Scottish Region of British Railways during the nationalisation of 1948. It was then closed by British Railways.

Preceding station	Historical railways			Following station
Locharbriggs Line and station closed		Dumfries, Lochmaben and Lockerbie Railway Caledonian Railway		Shieldhill Line and station closed

Some parts of the platform remain and the station house is now a private residence.

• Butt, R. V. J. (October 1995). The Directory of Railway Stations: details every public and private passenger station, halt, platform and stopping place, past and present (1st ed.). Sparkford: Patrick Stephens Ltd. ISBN 978-1-85260-508-7. OCLC 60251199. OL 11956311M.
• Jowett, Alan (2000). Jowett's Nationalised Railway Atlas (1st ed.). Penryn, Cornwall: Atlantic Transport Publishers. ISBN 978-0-906899-99-1. OCLC 228266687.
• Jowett, Alan (March 1989). Jowett's Railway Atlas of Great Britain and Ireland: From Pre-Grouping to the Present Day (1st ed.). Sparkford: Patrick Stephens Ltd. ISBN 978-1-85260-086-0. OCLC 22311137.
• Station on navigable O.S. map