Appleford Railway Bridge carries the Cherwell Valley Line from Didcot to Oxford across the River Thames near the village of Appleford-on-Thames, Oxfordshire, England. It crosses the Thames on the reach between Clifton Lock and Culham Lock.

Originally, a timber bridge was built at the approximate location of the present bridge; by December 1843, it was reportedly under construction, and was completed during the following year. By the 1850s, it has been replaced by a more substantial bridge principally composed of wrought iron so that heavier trains could be run along the line. A third bridge was completed in 1927, which was built out of steel; it is this structure that is presently used as of the 2010s.

• Crossings of the River Thames

• Appleford Railway Bridge via structurae.net
• Appleford Railway Bridge, River Thames via geograph.org.uk
• Appleford Railway Bridge via thames.me.uk

Next crossing upstream	River Thames	Next crossing downstream
Sutton Bridge (road)	Appleford Railway Bridge	Clifton Hampden Bridge (road)