Applegate Valley is the valley of the Applegate River in Southern Oregon, United States and extending slightly into Northern California. It encompasses the area between Applegate and Grants Pass, generally 20 miles (30 km) west of Medford. Oregon Route 238 (Jacksonville Highway) is a main route through the valley.

Many wineries of the Applegate Valley AVA (American Viticultural Area), a sub-appellation of the Rogue Valley AVA, are located in the valley.

The area is also a well known medical marijuana growing region.

• Applegate, Oregon
• Ruch, Oregon

• Applegate Valley Fire Plan: USDA

42°19′N 123°17′W / 42.32°N 123.29°W / 42.32; -123.29