The Baltimore and Ohio Related Industries Historic District comprises a portion of Martinsburg, West Virginia to either side of the Baltimore and Ohio Railroad line as it runs through the city. The district includes the Baltimore and Ohio Railroad Martinsburg Shops, a National Historic Landmark, and a variety of industrial and commercial concerns that depended on the railroad.

Along with buildings, the district includes the infrastructure associated with the building of the railroad in an urbanizing environment, such as the channelizing of Tuscarora Creek and a variety of culverts, underpasses and retaining walls. Significant buildings include, apart from the roundhouse/shop complex, the B. & O. Railroad station and hotel. Industrial buildings include coal yards, a plaster mill, feed mills, a distillery and other manufacturing facilities.

• Media related to Baltimore and Ohio and Related Industries Historic District at Wikimedia Commons
• Historic American Engineering Record (HAER) No. WV-1, "Baltimore & Ohio Railroad, Martinsburg Repair Shops, West Side of Tuscarora Creek Opposite East End of Race Street, Martinsburg, Berkeley County, WV", 11 photos, 4 data pages, 1 photo caption page
• HAER No. WV-1-A, "Baltimore & Ohio Railroad, Martinsburg West Roundhouse", 11 photos, 5 measured drawings, 2 data pages, 1 photo caption page
• HAER No. WV-1-B, "Baltimore & Ohio Railroad, Martinsburg East Roundhouse", 4 photos, 3 measured drawings, 2 data pages, 1 photo caption page
• HAER No. WV-1-C, "Baltimore & Ohio Railroad, Martinsburg Machine Shop", 3 photos, 1 photo caption page
• HAER No. WV-17, "Martinsburg Station & Hotel, Martin Street, Martinsburg, Berkeley County, WV", 1 photo, 1 photo caption page