Bernisse (Dutch pronunciation: [bɛrˈnɪsə] ) is a village and former municipality in the western Netherlands, in the province of South Holland. Since 2015 it has been a part of the municipality of Nissewaard.

The former municipality had a population of data missing in 2021, and covered an area of 68.46 km (26.43 sq mi) of which 11.12 km (4.29 sq mi) was water.

The former municipality was named after the river Bernisse, which flows right through it from the Spui to the Brielse Meer and separates the estuary islands of Voorne and Putten.

The former municipality of Bernisse consisted of the following communities: Abbenbroek, Biert, Geervliet, Heenvliet, Oudenhoorn, Simonshaven, Zuidland.

The municipality of Bernisse was formed on January 1, 1980, through the amalgamation of the municipalities Abbenbroek, Oudenhoorn, Zuidland, and parts of the municipalities Geervliet (including Simonshaven) and Heenvliet.

Dutch Topographic map of the former municipality of Bernisse, 2013.

• Dirk van Hogendorp (1761 in Heenvliet – 1822) a somewhat "troublesome" Dutch officer and author
• Maarten den Bakker (born 1969 in Abbenbroek) a retired road bicycle racer, competed in the 1988 Summer Olympics
• Alexander van Oudenaarden (1970 in Zuidland) a Dutch biophysicist and systems biologist
• Chanella Stougje (born 1996 in Zuidland) a Dutch professional racing cyclist

• Official website