The Diocese of Altamura-Gravina-Acquaviva delle Fonti (Latin: Dioecesis Altamurensis-Gravinensis-Aquavievensis) is a Latin Church diocese of the Catholic Church in Apulia, southern Italy, 40 km (25 miles) south-west of the coastal city of Bari. In 1986. the territorial prelature of Altamura e Acquaviva delle Fonti was united with the diocese of Gravina. The present diocese is a suffragan of the archdiocese of Bari-Bitonto.

The seat of the bishop is at Altamura Cathedral, with Acquaviva Cathedral and Gravina Cathedral as co-cathedrals.

Altamura was once a territorial prelature, founded in 1232 and endowed by the Emperor Frederick II, who declared it to be free and exempt from all episcopal jurisdiction. It was governed by an archpriest. It was declared exempt from episcopal jurisdiction by Pope Innocent IV in 1248, and again by Pope Innocent VIII (1484–92).

The Chapter of the major church of the territorial prelature of Altamura was composed of four dignities (the Archdeacon, the Cantor, the Primicerius, and the Treasurer) and twenty-four Canons, who had the right to use the cappa magna and rochet.

Acquaviva delle Fonti, a town of the Campagna, was declared similarly exempt by Pope Pius IX and united with Altamura on 17 August 1848.

Following the Second Vatican Council, and in accordance with the norms laid out in the council's decree, Christus Dominus chapter 40, It also recommended the abolition of anomalous units such as exempt territorial prelatures. Pope Paul VI ordered a reorganization of the ecclesiastical provinces in southern Italy, beginning with consultations among the members of the Congregation of Bishops in the Vatican Curia, the Italian Bishops Conference, and the various dioceses concerned.

On 18 February 1984, the Vatican and the Italian State signed a new and revised concordat. Based on the revisions, a set of Normae was issued on 15 November 1984, which was accompanied in the next year, on 3 June 1985, by enabling legislation. According to the agreement, the practice of having one bishop govern two separate dioceses at the same time, aeque personaliter, was abolished. The Vatican continued consultations which had begun under Pope John XXIII for the merging of small dioceses, especially those with personnel and financial problems, into one combined diocese.

On 30 September 1986, Pope John Paul II ordered that the diocese of Gravina be merged with the territorial prelatures of Altamura and of Aquaviva into one diocese with one bishop, with the Latin title Dioecesis Altamurensis-Gravinensis-Aquavivensis. The seat of the diocese was to be in Altamura, where the prelatial church was to serve as the cathedral of the merged diocese. The cathedral in Gravina and the prelatial seat in Aquaviva were to have the honorary titles of "co-cathedral"; the Chapters were each to be a Capitulum Concathedralis. There was to be only one diocesan Tribunal, in Altamura, and likewise one seminary, one College of Consultors, and one Priests' Council. The territory of the new diocese was to include the territory of the suppressed diocese and territorial prelatures. To make the territories of the new diocese congruent, the town of San Teramo in Colle was detached from the archdiocese of Bari-Bitonto and added to that of Altamura. The new diocese was made a suffragan of the archdiocese of Bari-Bitonto.

Erected: 1248
Latin Name: Altamurensis

Name Changed: 17 August 1848
Latin Name: Altamurensis et Aquavievensis
Metropolitan: Archdiocese of Bari-Canosa

• Salvatore Rotolo, S.D.B. (1948–1962 Retired)
• Antonio D'Erchia (1962–1964 Appointed Apostolic Administrator of Conversano)
• Salvatore Isgró (1975–1982 Appointed Archbishop of Sassari)
• Tarcisio Pisani, O.M. (1982–1986 Appointed Bishop of Altamura-Gravina-Acquaviva delle Fonti)

30 September 1986: United with the Diocese of Gravina
Latin Name: Altamurensis-Gravinensis-Aquavievensis

• Tarcisio Pisani, O.M. (30 Sep 1986-1994)
• Agostino Superbo (1994–1997 Resigned)
• Mario Paciello (1997–2013 Retired)
• Giovanni Ricchiuti (2013– )

• Roman Catholic Diocese of Gravina-Montepeloso

• Ciccimarra, Nicola (1964). La Cattedrale di Altamura: monumento di vita, di fede, di arte (in Italian). Altamura: Cressati.
• Cordasco, Pasquale, ed. (1994). Le pergamene della cattedrale di Altamura (1309-1381) (in Italian and Latin). Bari: Società di storia patria per la Puglia.
• D'Avino, Vincenzo (1848). Cenni storici sulle chiese arcivescovili, vescovili, e prelatizie (nulluis) del Regno delle Due Sicilie (in Italian). Napoli: Ranucci. pp. 748–749.
• Giaconella, Francesco (2020), "L’inedita cronaca di Altamura di Michele Santoro," Altamura: Rivista storica no. 61 (Altamura: Archivio-Biblioteca-Museo Civico, Altamura 2020), pp. 115–138, at p. 123.
• Giannone, Pietro (1821). Istoria civile del regno di Napoli (in Italian). Vol. quinto (5). Milano: Nicolò Bettoni. pp. 454–459.
• Giustiniani, Lorenzo (1797). Dizionario geografico ragionato del Regno di Napoli (in Italian). Vol. Tom. I. Napoli: Vincenzo Manfredi. pp. 125–136.
• Lucarelli A. (1904), Notizie e documenti riguardanti la storia di Acquaviva delle Fonti, (in Italian), Vol. I, Giovinazzo, 1904.
• Serena, Ottavio (1902). "La chiesa di Altamura, la serie dei suoi prelati e le sue iscrizioni", in: Rassegna pugliese di scienze lettere ed arti 19, nos. 11-12 (1902), pp. 322–337.

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