The Blood Hole massacre occurred in what is now the Australian state of Victoria at Middle Creek, 10–11 kilometres (6–7 mi) from Glengower Station between Clunes and Newstead at the end of 1839 or early 1840, killing an unknown number of Aboriginal people from the Grampians district who were on their way home after trading goods for green stone axe blanks that they obtained near what is now Lancefield.

Captain Dugald McLachlan established Glengower station, sometimes employing local Aboriginal people from the Dja Dja Wurrung (Jaara people). His employees also gave out flour and sugar rations to them on occasion.

After the cook distributed flour laced with plaster of Paris the party speared the cook in retribution, and ate the meat hanging in the kitchen. Later the Aboriginal people who had passed through on their way home were found at Middle Creek, a camping place on their trading route from the Grampians to the Greenstone quarry at Mount William near Lancefield.

The Aboriginal people were found at the waterhole on Middle Creek west of Glengower Station. They sought to hide by diving into the waterhole, where they were shot one at a time as they came up for air.

• Razik, Naveen (11 July 2020). "Victoria to introduce Australia's first truth-telling process to address Indigenous injustices". SBS News, Special Broadcasting Service. Archived from the original on 11 July 2020. Retrieved 11 July 2020.
• Thorne, Leonie (11 July 2020). "Victoria to establish truth and justice process as part of Aboriginal treaty process". ABC News. Australian Broadcasting Corporation. Archived from the original on 11 July 2020. Retrieved 11 July 2020.