Botanic railway station serves the Botanic area in south Belfast, Northern Ireland and students for Queen's University Belfast; it is also near Shaftesbury Square which is along Botanic Avenue. It is named after the nearby Belfast Botanic Gardens. It is one of the four stations located in the city centre, the others being City Hospital, Lanyon Place, and the under-construction Grand Central.

The station opened on 26 April 1976 and is very close to City Hospital Station

Passengers can alight here for the Ulster Museum, which is situated on the edge of Botanic Gardens.

On weekdays, there is a half-hourly service on the Bangor Line between Grand Central and Bangor, with extra trains at peak times. The Derry~Londonderry Line operates an hourly service between Grand Central and Derry~Londonderry, with additional trains to Coleraine at peak times. The Larne Line operates a half-hourly service to Larne Harbour and Whitehead, with the terminus alternating between the two every half-hour. Extra trains operate to Carrickfergus and Larne Town at peak times.

On Saturdays, service patterns remain largely similar except without any additional peak-time trains.

On Sundays, the Bangor Line reduces to an hourly service. The Derry~Londonderry Line alternates its outbound terminus every hour between Derry~Londonderry and Portrush, resulting in a two-hourly service to stations beyond Coleraine. The Larne Line also reduces to hourly but continues alternating the terminus between Larne Harbour and Whitehead, resulting in a two-hourly service to stations beyond Whitehead.

Preceding station		Northern Ireland Railways		Following station
City Hospital		Northern Ireland Railways Belfast-Derry		Lanyon Place
		Northern Ireland Railways Belfast-Larne
		Northern Ireland Railways Belfast-Bangor

The timetable allows 3 minutes for the trains to travel between Lanyon Place and Botanic.

At one point, the cross-border Enterprise service to Dublin Connolly served Botanic, but no longer stops at the station.

Belfast Metro's 7 services connect Botanic station to the City Centre, Four Winds, Laurelgrove, Braniel, and Stormont. Additionally, the 625 Ulsterbus service also stop near the station with runs from the City Centre to Ballynahinch and Carryduff. There are no ticket vending machines (TVMs) for bus tickets; passengers can either download the mLink app or pay directly on the bus.

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