The Diocese of Brno (Latin: Dioecesis Brunensis) is a Latin diocese located in the city of Brno in the ecclesiastical province of Olomouc in the Czech Republic.

• 1296: A collegiate (latter the cathedral) chapter established in Brno
• 1625: A collegiate chapter established in Mikulov
• 1777, December 5: The Diocese established from a part of the Diocese of Olomouc
• 1780s: As a consequence of the Edict on Idle Institutions (by Joseph II) some ancient monasteries and convents abolished
• 1783: Territory (substantially) extended
• 1807: A seminary established
• 1909: The first diocesan synod was held
• 1934: The second diocesan synod was held
• 1938-1945: Substantial southern part of the diocese (under the rule of Nazi Germany) administered separately by vicar general in Mikulov
• 1945-1946: German population expelled from the diocese
• 1950: The seminary abolished due to the Communist rule
• 1950-1968: Communists prevented Bishop Karel Skoupý from tenure of office
• 1972-1990: Sede vacante due to the Communist rule
• 1999: Administrative reorganisation: number of deaneries reduced from 37 to 20

• Basilica of the Assumption in Staré Brno
• Basilica of Our Lady of the Assumption and St. Nicholas in Žďár nad Sázavou
• St. Wenceslaus Collegiate Church in Mikulov

• Mathias Franz Chorinsky von Ledske (1777–1786)
• Johann Baptist Lachenbauer, OCr (1786–1799)
• Vinzenz Joseph Franz Sales von Schrattenbach (1800–1816)
• Wenzel Urban von Stuffler (1817–1831)
• Franz Anton Gindl (1831–1841)
• Anton Ernst von Schaffgotsche (1841–1870)
• Karl Nöttig (1870–1882)
• František Saleský Cardinal Bauer (1882–1904)
• Pavel Huyn (1904–1916)
• Norbert Johann Klein, OT (1916–1926)
• Josef Kupka (1931–1941)
• Karel Skoupý (1946–1972)
• Vojtěch Cikrle (1990-2022)
• Pavel Konzbul (since 2022)

• St. Peter and Paul, patron saints of the diocese
• St. Zdislava, born 1220s in Křižanov
• St. Clement Hofbauer, born 1751 in Tasovice
• Blessed Maria Restituta, born 1894 in Husovice (now part of Brno)
• Jan Bula, a Catholic priest executed by the Communist regime in 1952. The Diocese has been processing his beatification since 2004.

• Roman Catholicism in the Czech Republic

• Katalog brněnské diecéze. Neproměnná část. Brno 2007.
• GCatholic.org
• Catholic Hierarchy
• Diocese website

49°12′00″N 16°36′00″E / 49.2000°N 16.6000°E / 49.2000; 16.6000