The Roman Catholic Diocese of Kwito-Bié (Latin: Dioecesis Kvitobiensis) is a diocese located in Bié Province in the ecclesiastical province of Huambo in Angola. The cathedral is located in the city of Cuito.

The diocese was established on 4 September 1940 as the Diocese of Silva Porto on territory separated from the Diocese of São Paulo de Loanda. Initially, the diocese was a suffragan of the Roman Catholic Archdiocese of Luanda. The diocese lost territory on 25 November 1957 to establish the Roman Catholic Diocese of Malanje and on 1 July 1963 to establish the Diocese of Luso.

On 3 February 1977, the diocese changed suffragans to the Archdiocese of Huambo. On 16 May 1979, it was renamed as the Diocese of Kwito-Bié.

The Cathedral of the diocese is Sé Catedral de São Lourenço in Cuito, Bié Province.

• Bishop-elect Vicente Sanombo (28 March 2024 – )
• Bishop José Nambi (15 January 1997 – 31 October 2022)
• Bishop Pedro Luís António (15 June 1979 – 15 January 1997)
• Bishop Manuel António Pires (16 May 1979 – 15 June 1979); see below

• Bishop Manuel António Pires (23 September 1958 – 16 May 1979); see above
• Bishop Antonio Ildefonse dos Santos Silva, O.S.B. (3 November 1941 – 17 August 1958)

• Manuel António Pires (1955-1958)
• José Nambi (1995-1997)

• Roman Catholicism in Angola

• GCatholic.org
• "Diocese of Kwito-Bié". Catholic-Hierarchy.org. David M. Cheney.

12°23′03″S 16°56′39″E / 12.3843°S 16.9441°E / -12.3843; 16.9441