Ein Ya'akov (Hebrew: עֵין יַעֲקֹב, lit. 'Jacob's spring') is a moshav in northern Israel. Located near Ma'alot-Tarshiha, it falls under the jurisdiction of Ma'ale Yosef Regional Council. In 2022 it had a population of 1,226.

The village was established on 15 October 1950 by immigrants from Kurdistan and Iraq. Its name (as of the neighbouring moshav Me'ona) is taken from Deuteronomy 33:27–28:

And Israel dwelleth in safety, Jacob's spring is secure, in a land of corn and wine; yea, his heavens drop down dew.