The Espíritu Santo River is a Bolivian river in the Cochabamba Department, Chapare Province, Villa Tunari Municipality. It belongs to the Amazon River watershed. Espíritu Santo River originates in the Callejas mountains south west of Villa Tunari. It initially flows in a northeasterly direction, before turning to the east towards Villa Tunari. In the east of the town, it meets San Matéo River which comes from the south west. From the confluence the river is known as Chapare River.

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  San Matéo River and Espíritu Santo River confluence to form Chapare River at Villa Tunari

• List of rivers of Bolivia

• lib.utexas.edu Detailed map of the area
• Rand McNally, The New International Atlas, 1993.

• Media related to Espíritu Santo River at Wikimedia Commons