File:Opthalmology AMD Super Resolution Cremer.png
Summary
| Description |
English: Investigation of AMD, macular degeneration a medical condition which usually affects older adults and results in a loss of vision in the center of the visual field (the macula) because of damage to the retina macular degeneration.
Former Lab of Christoph Cremer, emeritus at Heidelberg UniversitySections from human eye tissue were analyzed with Structured Illumination Microscopy (SMI) / Optical nanoscopy using a specially designed Super Resolution Microscope setup: LSI-TIRF as a total internal reflection interferometer with laterally structured illumination. This SMI technique allowed to acquire light-optical images of autofluorophore distributions in the tissue with previously unmatched optical resolution. Use of three different excitation wavelengths (488, 568 and 647 nm), enables to gather spectral information about the autofluorescence signal. Detailed Image description: Overview image. The SMI image (right) shows more details and less out of focus light than the widefield image (left). The Bruch membrane (B) is located between the choroid (C) and the retinal pigment epithelium (RPE). On the top of the image endings of retinal rod cells (R) can be seen. The scale bar is 2�m. Reference: Best G, Amberger R, Baddeley D, Ach T, Dithmar S, Heintzmann R and Cremer C (2011). Structured illumination microscopy of autofluorescent aggregations in human tissue. Micron, 42, 330-335. |
| Date | |
| Source | Own work |
| Author | Andy Nestl |
Gallery
- Super Resolution Microscopy - Localisation Microscopy + Structured Illumination
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Breast Cancer Cells: 3D Dual Color Super Resolution Microscopy of Her2 and Her3 & cluster calculations -
Single YFP molecule detection in a human cancer cell. Typical distance measurements 15 nm -
Co- localisation microscopy with GFP and RFP fusion proteins (nucleus of a bone cancer cell) 120.000 localized molecules in a widefield area(470 µm2) -
Label-free Localisation Microscopy SPDM - Super Resolution Microscopy reveals prior undetebable intracellular structures -
Investigation of human eye tissue, affected by macular degeneration -
Virus Super Resolution Microscopy SPDM Cremer/Wege labs
Licensing
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- ↑ https://www.physik.uni-heidelberg.de/personen/lsf.php?details=1537 |titel=Fakultät für Physik und Astronomie |abruf=2020-10-01
