The Chicago and Northwestern Railroad Depot is a historic former railroad station in Fond du Lac, Wisconsin. The station is located on the southeast corner of Forest Avenue and Brooke Street.

Rail service in Fond du Lac can be traced as far back as 1852, although Fond du Lac was also the namesake of the former Chicago, St. Paul and Fond du Lac Railroad, which existed from 1855 to 1859 before going bankrupt and being acquired by the Chicago and Northwestern Railway. In 1891 C&NW replaced the original station with the current one. The building's architect was Charles Sumner Frost. A near-identical twin station was built, also in 1891, in DeKalb, Illinois.

The station served trains such as the Flambeau 400 and Peninsula 400 until it was closed in 1975, well after most intercity passenger service in the United States was turned over to Amtrak in 1971, which never used this station.

The Chicago and Northwestern Railroad Depot was added to the National Register of Historic Places on August 10, 1990. Today it houses several small shops, and the former right-of-way is a rail trail. An active railroad line exists west of the station, but that line was operated by Soo Line and no longer contains a station nearby.

• Fond du Lac Train Depot (Facebook)

Preceding station	Chicago and North Western Railway			Following station
Oshkosh toward Ishpeming		Ishpeming – Milwaukee		Eden toward Milwaukee
Oakfield toward Janesville		Janesville – Fond du Lac		Terminus
Rosendale toward Marshfield		Marshfield – Fond du Lac
Terminus		Fond du Lac – Sheboygan		Peeble's toward Sheboygan