Gladstone railway station is located on the Crystal Brook-Broken Hill line in Gladstone, South Australia.

Gladstone station opened in 1876 when a line opened from Port Pirie in the west, it was later extended east to Peterborough and ultimately Broken Hill. In 1888, a line was built north to Laura and ultimately Wilmington. When the Hamley Bridge line from Balaklava in the south reached Gladstone in 1894, it became a four-way junction station. All were built as narrow gauge lines.

In 1927, the line from the south was converted to broad gauge, making Gladstone a break of gauge station. As part of the standardisation project, the line between Port Augusta and Broken Hill was converted to standard gauge in 1969, thus Gladstone became a junction for three gauges.

The broad-gauge connection between Gladstone and Adelaide was closed in May 1988. The narrow-gauge Wilmington railway line was closed in 1990 thus making Gladstone an only standard gauge station on the Crystal Brook-Broken Hill railway line.

Journey Beyond's weekly Indian Pacific service between Sydney and Perth passes through Gladstone but does not stop.

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