Hester Brook is a rural locality of the Shire of Bridgetown–Greenbushes in the South West region of Western Australia. Hester Brook is located on the South Western Highway, which passes through its north-eastern part. The Blackwood River forms the south-western border of the locality.

The Shire of Bridgetown–Greenbushes is located on the traditional land of the Bibulman (also spelled Bibbulmun or Pibelmen) and Wardandi (also spelled Wadandi) people, both of the Noongar nation.

The heritage-listed Peninsula House, a homestead dating back to 1920, is located in Hester Brook, overlooking a bend in the Blackwood River. An earlier cottage at the location, dating back to 1890, was destroyed in a bushfire in 2009. A second heritage-listed building, Parkville, also overlooking a bend in the river, dates back to 1900. An older dwelling at the site, built a decade earlier, is now used as a tool shed.