The largest find of Iguanodon remains to that date occurred on 28 February 1878 in a coal mine at Bernissart in Belgium, at a depth of 322 m (1,056 ft), when two mineworkers, Jules Créteur and Alphonse Blanchard, accidentally hit on a skeleton that they initially took for petrified wood. With the encouragement of Alphonse Briart, supervisor of mines at nearby Morlanwelz, Louis de Pauw on 15 May 1878 started to excavate the skeletons and in 1882 Louis Dollo reconstructed them. At least 38 Iguanodon individuals were uncovered, most of which were adults. In 1882, the holotype specimen of I. bernissartensis became one of the first ever dinosaur skeletons mounted for display. It was put together in a chapel at the Palace of Charles of Lorraine using a series of adjustable ropes attached to scaffolding so that a lifelike pose could be achieved during the mounting process. This specimen, along with several others, first opened for public viewing in an inner courtyard of the palace in July 1883. In 1891 they were moved to the Royal Museum of Natural History, where they are still on display; nine are displayed as standing mounts, and nineteen more are still in the Museum's basement. The exhibit makes an impressive display in the Royal Belgian Institute of Natural Sciences, in Brussels. A replica of one of these is on display at the Oxford University Museum of Natural History and at the Sedgwick Museum in Cambridge. Most of the remains were referred to a new species, I. bernissartensis, a larger and much more robust animal than the English remains had yet revealed. One specimen, IRSNB 1551, was at first referred to the nebulous, gracile I. mantelli, but is currently referred to Mantellisaurus atherfieldensis. The skeletons were some of the first complete dinosaur skeletons known. Found with the dinosaur skeletons were the remains of plants, fish, and other reptiles, including the crocodyliform Bernissartia.

The science of conserving fossil remains was in its infancy, and new techniques had to be improvised to deal with what soon became known as "pyrite disease". Crystalline pyrite in the bones was being oxidized to iron sulphate, accompanied by an increase in volume that caused the remains to crack and crumble. When in the ground, the bones were isolated by anoxic moist clay that prevented this from happening, but when removed into the drier open air, the natural chemical conversion began to occur. To limit this effect, De Pauw immediately, in the mine-gallery, re-covered the dug-out fossils with wet clay, sealing them with paper and plaster reinforced by iron rings, forming in total about six hundred transportable blocks with a combined weight of a hundred and thirty tons. In Brussels after opening the plaster he impregnated the bones with boiling gelatine mixed with oil of cloves as a preservative. Removing most of the visible pyrite he then hardened them with hide glue, finishing with a final layer of tin foil. Damage was repaired with papier-mâché. This treatment had the unintended effect of sealing in moisture and extending the period of damage. In 1932 museum director Victor van Straelen decided that the specimens had to be completely restored again to safeguard their preservation. From December 1935 to August 1936 the staff at the museum in Brussels treated the problem with a combination of alcohol, arsenic, and 390 kilograms of shellac. This combination was intended to simultaneously penetrate the fossils (with alcohol), prevent the development of mold (with arsenic), and harden them (with shellac). The fossils entered a third round of conservation from 2003 until May 2007, when the shellac, hide glue and gelatine were removed and impregnated with polyvinyl acetate and cyanoacrylate and epoxy glues. Modern treatments of this problem typically involve either monitoring the humidity of fossil storage, or, for fresh specimens, preparing a special coating of polyethylene glycol that is then heated in a vacuum pump, so that moisture is immediately removed and pore spaces are infiltrated with polyethylene glycol to seal and strengthen the fossil.

Dollo's specimens allowed him to show that Owen's prehistoric pachyderms were not correct for Iguanodon. He instead modelled the skeletal mounts after the cassowary and wallaby, and put the spike that had been on the nose firmly on the thumb. His reconstruction would prevail for a long period of time, but would later be discounted.

Excavations at the quarry were stopped in 1881, although it was not exhausted of fossils, as recent drilling operations have shown. During World War I, when the town was occupied by German forces, preparations were made to reopen the mine for palaeontology, and Otto Jaekel was sent from Berlin to supervise. Just as the first fossiliferous layer was about to be uncovered, however, the German army surrendered and had to withdraw. Further attempts to reopen the mine were hindered by financial problems and were stopped altogether in 1921 when the mine flooded.

Research on Iguanodon decreased during the early part of the 20th century as World Wars and the Great Depression enveloped Europe. A new species that would become the subject of much study and taxonomic controversy, I. atherfieldensis, was named in 1925 by R. W. Hooley, for a specimen collected at Atherfield Point on the Isle of Wight.

Iguanodon was not part of the initial work of the dinosaur renaissance that began with the description of Deinonychus in 1969, but it was not neglected for long. David B. Weishampel's work on ornithopod feeding mechanisms provided a better understanding of how it fed, and David B. Norman's work on numerous aspects of the genus has made it one of the best-known dinosaurs. In addition, a further find of numerous disarticulated Iguanodon bones in Nehden, Nordrhein-Westphalen, Germany, has provided evidence for gregariousness in this genus, as the animals in this areally restricted find appear to have been killed by flash floods. At least 15 individuals, from 2 to 8 metres (6 ft 7 in to 26 ft 3 in) long, have been found here, most of the individuals belong to the related Mantellisaurus (described as I. atherfieldensis, at that time believed to be another species of Iguanodon). but some are of I. bernissartensis.

One major revision to Iguanodon brought by the Renaissance would be another re-thinking of how to reconstruct the animal. A major flaw with Dollo's reconstruction was the bend he introduced into the tail. This organ was more or less straight, as shown by the skeletons he was excavating, and the presence of ossified tendons. In fact, to get the bend in the tail for a more wallaby or kangaroo-like posture, the tail would have had to be broken. With its correct, straight tail and back, the animal would have walked with its body held horizontal to the ground, arms in place to support the body if needed.

In the 21st century, Iguanodon material has been used in the search for dinosaur biomolecules. In research by Graham Embery et al., Iguanodon bones were processed to look for remnant proteins. In this research, identifiable remains of typical bone proteins, such as phosphoproteins and proteoglycans, were found in a rib. In 2007, Gregory S. Paul split I. atherfieldensis into a new, separate genus, Mantellisaurus which has been generally accepted. In 2009 fragmentary iguanodontid material was described from upper Barremian Paris Basin deposits in Auxerre, Burgundy. While not definitively diagnosable to the genus/species level, the specimen shares "obvious morphological and dimensional affinities" with I. bernissartensis.

In 2010, David Norman split the Valanginian species I. dawsoni and I. fittoni into Barilium and Hypselospinus respectively. After Norman 2010, over half a dozen new genera were named off English "Iguanodon" material. Carpenter and Ishida in 2010 named Proplanicoxa, Torilion and Sellacoxa while Gregory S. Paul in 2012 named Darwinsaurus, Huxleysaurus and Mantellodon and Macdonald et al. in 2012 named Kukufeldia. These species named after Norman 2010 are not considered valid and are considered various junior synonyms of Mantellisaurus, Barilium and Hypselospinus.

In 2011, a new genus Delapparentia was named for a specimen in Spain that was originally thought to belong to I. bernissartensis. The previous identification was subsequently reaffirmed in a new analysis of individual variation in the Belgian specimens, finding that the Delapparentia specimen was within the range of I. bernissartensis. In 2015 a new species of Iguanodon, I. galvensis, was named based on material including 13 juvenile (perinate) individuals found in the Camarillas Formation near Galve, Spain. In 2017 a new study was done of I. galvensis, with further evidence of distinctiveness from I. bernissartensis including several new autapomorphies. It was also found that the Delapparentia holotype (which is also from the Camarillas Formation) was not distinguishable from either I. bernissartensis or I. galvensis.

Iguanodon were bulky herbivores that could shift from bipedality to quadrupedality. The only well-supported species, I. bernissartensis, is estimated to have measured about 9 metres (30 feet) long as an adult, with some specimens possibly as long as 13 metres (43 feet), although this is likely an overestimate, given that the maximum body length of I. bernissartensis is reported to be 11 m (36 ft). Although Gregory S. Paul suggested a body mass of 3.08 metric tons (3.40 short tons) on average, constructing a 3D mathematical model and employing allometry-based estimate suggests an I. bernissartensis close to 8 m (26 ft) long (smaller than average) weighs close to 3.8 metric tons (4.2 short tons) in body mass. Specimens of relatively large individuals have been reported in the 2020s: a specimen referred to as I. cf. galvensis was measured up to 9–10 m (30–33 ft) in length, while a new specimen of I. bernissartensis from the upper Barremian of the Iberian Peninsula was measured up to 11 m (36 ft) in length. Such large individuals would have weighed approximately 4.5 metric tons (5.0 short tons).

The arms of I. bernissartensis were long (up to 75% the length of the legs) and robust, with rather inflexible hands built so that the three central fingers could bear weight. The thumbs were conical spikes that stuck out away from the three main digits. In early restorations, the spike was placed on the animal's nose. Later fossils revealed the true nature of the thumb spikes, although their exact function is still debated. They could have been used for defense, or for foraging for food. The little finger was elongated and dextrous, and could have been used to manipulate objects. The phalangeal formula is 2-3-3-2-4, meaning that the innermost finger (phalange) has two bones, the next has three, etc. The legs were powerful, but not built for running, and each foot had three toes. The backbone and tail were supported and stiffened by ossified tendons, which were tendons that turned to bone during life (these rod-like bones are usually omitted from skeletal mounts and drawings).

These animals had large, tall but narrow skulls, with toothless beaks probably covered with keratin, and teeth like those of iguanas, as the name suggests, but much larger and more closely packed. Unlike hadrosaurids, which had columns of replacement teeth, Iguanodon only had one replacement tooth at a time for each position. The upper jaw held up to 29 teeth per side, with none at the front of the jaw, and the lower jaw 25; the numbers differ because teeth in the lower jaw are broader than those in the upper. Because the tooth rows are deeply inset from the outside of the jaws, and because of other anatomical details, it is believed that, as with most other ornithischians, Iguanodon had some sort of cheek-like structure, muscular or non-muscular, to retain food in the mouth.

Iguanodon gives its name to the unranked clade Iguanodontia, a very populous group of ornithopods with many species known from the Middle Jurassic to the Late Cretaceous. Aside from Iguanodon, the best-known members of the clade include Dryosaurus, Camptosaurus, Ouranosaurus, and the duck-bills, or hadrosaurs. In older sources, Iguanodontidae was shown as a distinct family. This family traditionally has been something of a wastebasket taxon, including ornithopods that were neither hypsilophodontids or hadrosaurids. In practice, animals like Callovosaurus, Camptosaurus, Craspedodon, Kangnasaurus, Mochlodon, Muttaburrasaurus, Ouranosaurus, and Probactrosaurus were usually assigned to this family.

With the advent of cladistic analyses, Iguanodontidae as traditionally construed was shown to be paraphyletic, and these animals are recognised to fall at different points in relation to hadrosaurs on a cladogram, instead of in a single distinct clade. Essentially, the modern concept of Iguanodontidae currently includes only Iguanodon. Groups like Iguanodontoidea are still used as unranked clades in the scientific literature, though many traditional iguanodontids are now included in the superfamily Hadrosauroidea. Iguanodon lies between Camptosaurus and Ouranosaurus in cladograms, and is probably descended from a camptosaur-like animal. At one point, Jack Horner suggested, based mostly on skull features, that hadrosaurids actually formed two more distantly related groups, with Iguanodon on the line to the flat-headed hadrosaurines, and Ouranosaurus on the line to the crested lambeosaurines, but his proposal has been rejected.

The cladogram below follows an analysis by Andrew McDonald, 2012.

Because Iguanodon is one of the first dinosaur genera to have been named, numerous species have been assigned to it. While never becoming the wastebasket taxon several other early genera of dinosaurs (such as Megalosaurus) became, Iguanodon has had a complicated history, and its taxonomy continues to undergo revisions. Although Gregory Paul recommended restricting I. bernissartensis to the famous sample from Bernissart, ornithopod workers like Norman and McDonald have disagreed with Paul's recommendations, except exercising caution when accepting records of Iguanodon from France and Spain as valid.

I. anglicus was the original type species, but the lectotype was based on a single tooth and only partial remains of the species have been recovered since. In March 2000, the International Commission on Zoological Nomenclature changed the type species to the much better known I. bernissartensis, with the new holotype being IRSNB 1534. The original Iguanodon tooth is held at Te Papa Tongarewa, the national museum of New Zealand in Wellington, although it is not on display. The fossil arrived in New Zealand following the move of Gideon Mantell's son Walter there; after the elder Mantell's death, his fossils went to Walter.

Only two species assigned to Iguanodon are still considered to be valid.

• I. bernissartensis, described by George Albert Boulenger in 1881, is the type species for the genus. This species is best known for the many skeletons discovered in the Sainte-Barbe Clays Formation at Bernissart, but is also known from remains across Europe.
  • Delapparentia turolensis, named in 2011 based on a specimen previously assigned to Iguanodon bernissartensis, was argued to be distinct from the latter based on the relative height of its neural spines. However, a 2017 study noted that this is easily within the range of individual variation, and that the difference may also arise from D. turolensis being an adult older than other specimens of I. bernissartensis.
  • I. seelyi (also incorrectly spelled I. seeleyi), described by John Hulke in 1882, has also been synonymised with Iguanodon bernissartensis, though this is not universally accepted. It was discovered in Brook, on the Isle of Wight, and named after Charles Seely MP, Liberal politician and philanthropist, on whose estate it was found.
  • David Norman has suggested that I. bernissartensis includes the dubious Mongolian I. orientalis (see also below), but this has not been followed by other researchers.
• I. galvensis, described in 2015, is based on adult and juvenile remains found in Barremian-age deposits in Teruel, Spain.

• I. albinus (or Albisaurus scutifer), described by Czech palaeontologist Antonin Fritsch in 1893, is a dubious nondinosaurian reptile now known as Albisaurus albinus.
• I. atherfieldensis, described by R.W. Hooley in 1925, was smaller and less robust than I. bernissartensis, with longer neural spines. It was renamed Mantellisaurus atherfieldensis in 2007. The Bernissart specimen RBINS 1551 was described as Dollodon bampingi in 2008, but McDonald and Norman returned Dollodon to synonymy with Mantellisaurus.
• I. dawsoni, described by Lydekker in 1888, is known from two partial skeletons found in East Sussex, England, from the middle Valanginian-age Lower Cretaceous Wadhurst Clay. It is now the type species of Barilium.
• I. exogyrarum was described by Fritsch in 1878. It is a nomen dubium based on very poor material and was renamed Ponerosteus in 2000.
• I. fittoni was described by Lydekker in 1889. Like I. dawsoni, this species was described from the Wadhurst Clay of East Sussex. It is now the type species of Hypselospinus.
• I. hilli, coined by Edwin Tully Newton in 1892 for a tooth from the early Cenomanian Upper Cretaceous Lower Chalk of Hertfordshire, has been considered an early hadrosaurid of some sort. However, recent work places it as indeterminate beyond Hadrosauroidea outside Hadrosauridae.
• I. hoggi (also spelled I. boggii or hoggii), named by Owen for a lower jaw from the Tithonian–Berriasian-age Upper Jurassic–Lower Cretaceous Purbeck Beds of Dorset in 1874, has been reassigned to its own genus, Owenodon.
• I. hollingtoniensis (also spelled I. hollingtonensis), described by Lydekker in 1889, has variously been considered a synonym of Hypselospinus fittoni or a distinct species assigned to the genus Huxleysaurus. A specimen from the Valanginian Wadhurst Clay Formation, variously assigned to I. hollingtoniensis and I. mantelli over the years, has an unusual combination of hadrosaurid-like lower jaw and very robust forelimb; Norman (2010) assigned this specimen to the species Hypselospinus fittoni, while Paul (2012) made it the holotype of a separate species Darwinsaurus evolutionis.
• I. lakotaensis was described by David B. Weishampel and Philip R. Bjork in 1989. The only well-accepted North American species of Iguanodon, I. lakotaensis was described from a partial skull from the Barremian-age Lower Cretaceous Lakota Formation of South Dakota. Its assignment has been controversial. Some researchers suggest that it was more basal than I. bernissartensis, and related to Theiophytalia, but David Norman has suggested that it was a synonym of I. bernissartensis. Gregory S. Paul has since given the species its own genus, Dakotadon.
• I. mantelli described by Christian Erich Hermann von Meyer in 1832, was based on the same material as I. anglicus and is an objective junior synonym of the latter. Several taxa, including the holotype of Mantellisaurus and Mantellodon, but also the dubious hadrosauroid Trachodon cantabrigiensis the hypsilophodont Hypsilophodon, and Valdosaurus, were previously mis-assigned to I. mantelli.
• "I. mongolensis" is a nomen nudum from a photo caption in a book by Whitfield in 1992 of remains that would later be named Altirhinus.
• I. orientalis, described by A. K. Rozhdestvensky in 1952, was based on poor material, but a skull with a distinctive arched snout that had been assigned to it was renamed Altirhinus kurzanovi in 1998. At the same time, I. orientalis was considered to be a nomen dubium because it cannot be compared to I. bernissartensis.
• I. phillipsi was described by Harry Seeley in 1869, but he later reassigned it to Priodontognathus.
• I. praecursor (also spelled I. precursor), described by E. Sauvage in 1876 from teeth from an unnamed Kimmeridgian (Late Jurassic) formation in Pas-de-Calais, France, is actually a sauropod, sometimes assigned to Neosodon, although the two come from different formations.
• I. prestwichii (also spelled I. prestwichi), described by John Hulke in 1880, has been reassigned to Camptosaurus prestwichii or to its own genus Cumnoria.
• I. suessii, described by Emanuel Bunzel in 1871, has been reassigned to Mochlodon suessi.

• I. foxii (also spelled I. foxi) was originally described by Thomas Henry Huxley in 1869 as the type species of Hypsilophodon; Owen (1873 or 1874) reassigned it to Iguanodon, but his assignment was soon overturned.
• I. gracilis, named by Lydekker in 1888 as the type species of Sphenospondylus and assigned to Iguanodon in 1969 by Rodney Steel, has been suggested to be a synonym of Mantellisaurus atherfieldensis, but is considered dubious nowadays.
• I. major, a species named by Justin Delair in 1966, based on vertebrae from the Isle of Wight and Sussex originally described by Owen in 1842 as a species of Streptospondylus, S. major, is a nomen dubium.
• I. valdensis, a renaming of Vectisaurus valdensis by Ernst van den Broeck in 1900. Originally named by Hulke as a distinct genus in 1879 based on vertebral and pelvic remains, it was from the Barremian stage of the Isle of Wight. It was considered a juvenile specimen of Mantellisaurus atherfieldensis, or an undetermined species of Mantellisaurus, but is indeterminate beyond Iguanodontia.
• The nomen nudum "Proiguanodon" (van den Broeck, 1900) also belongs here.

• I. anglicus, described by Friedrich Holl in 1829, is the original type species of Iguanodon, but, as discussed above, was replaced by I. bernissartensis. In the past, it has been spelled as I. angelicus (Lessem and Glut, 1993) and I. anglicum (Holl, 1829 emend. Bronn, 1850). It is possible teeth ascribed to this species belong to the genus now called Barilium. The name Therosaurus (Fitzinger, 1840), is a junior objective synonym, a later name for the material of I. anglicus.
• I. ottingeri, described by Peter Galton and James A. Jensen in 1979, is a nomen dubium based on teeth from the possibly Aptian-age lower Cedar Mountain Formation of Utah.

One of the first details noted about Iguanodon was that it had the teeth of a herbivorous reptile, although there has not always been consensus on how it ate. As Mantell noted, the remains he was working with were unlike any modern reptile, especially in the toothless, scoop-shaped form of the lower jaw symphysis, which he found best compared to that of the two-toed sloth and the extinct ground sloth Mylodon. He also suggested that Iguanodon had a prehensile tongue which could be used to gather food, like a giraffe. More complete remains have shown this to be an error; for example, the hyoid bones that supported the tongue are heavily built, implying a muscular, non-prehensile tongue used for moving food around in the mouth. The giraffe-tongue idea has also been incorrectly attributed to Dollo via a broken lower jaw.

The skull was structured in such a way that as it closed, the bones holding the teeth in the upper jaw would bow out. This would cause the lower surfaces of the upper jaw teeth to rub against the upper surface of the lower jaw's teeth, grinding anything caught in between and providing an action that is the rough equivalent of mammalian chewing. Because the teeth were always replaced, the animal could have used this mechanism throughout its life, and could eat tough plant material. Additionally, the front ends of the animal's jaws were toothless and tipped with bony nodes, both upper and lower, providing a rough margin that was likely covered and lengthened by a keratinous material to form a cropping beak for biting off twigs and shoots. Its food gathering would have been aided by its flexible little finger, which could have been used to manipulate objects, unlike the other fingers.

Exactly what Iguanodon ate with its well-developed jaws is not known. The size of the larger species, such as I. bernissartensis, would have allowed them access to food from ground level to tree foliage at 4–5 metres (13–16 ft) high. A diet of horsetails, cycads, and conifers was suggested by David Norman, although iguanodonts in general have been tied to the advance of angiosperm plants in the Cretaceous due to the dinosaurs' inferred low-browsing habits. Angiosperm growth, according to this hypothesis, would have been encouraged by iguanodont feeding because gymnosperms would be removed, allowing more space for the weed-like early angiosperms to grow. The evidence is not conclusive, though. Whatever its exact diet, due to its size and abundance, Iguanodon is regarded as a dominant medium to large herbivore for its ecological communities. In England, this included the small predator Aristosuchus, larger predators Eotyrannus, Baryonyx, and Neovenator, low-feeding herbivores Hypsilophodon and Valdosaurus, fellow "iguanodontid" Mantellisaurus, the armoured herbivore Polacanthus, and sauropods like Pelorosaurus.

Early fossil remains were fragmentary, which led to much speculation on the posture and nature of Iguanodon. Iguanodon was initially portrayed as a quadrupedal horn-nosed beast. However, as more bones were discovered, Mantell observed that the forelimbs were much smaller than the hindlimbs. His rival Owen was of the opinion it was a stumpy creature with four pillar-like legs. The job of overseeing the first lifesize reconstruction of dinosaurs was initially offered to Mantell, who declined due to poor health, and Owen's vision subsequently formed the basis on which the sculptures took shape. Its bipedal nature was revealed with the discovery of the Bernissart skeletons. However, it was depicted in an upright posture, with the tail dragging along the ground, acting as the third leg of a tripod.

During his re-examination of Iguanodon, David Norman was able to show that this posture was unlikely, because the long tail was stiffened with ossified tendons. To get the tripodal pose, the tail would literally have to be broken. Putting the animal in a horizontal posture makes many aspects of the arms and pectoral girdle more understandable. For example, the hand is relatively immobile, with the three central fingers grouped together, bearing hoof-like phalanges, and able to hyperextend. This would have allowed them to bear weight. The wrist is also relatively immobile, and the arms and shoulder bones robust. These features all suggest that the animal spent time on all fours.

Furthermore, it appears that Iguanodon became more quadrupedal as it got older and heavier; juvenile I. bernissartensis have shorter arms than adults (60% of hindlimb length versus 70% for adults). When walking as a quadruped, the animal's hands would have been held so that the palms faced each other, as shown by iguanodontian trackways and the anatomy of this genus's arms and hands. The three-toed pes (foot) of Iguanodon was relatively long, and when walking, both the hand and the foot would have been used in a digitigrade fashion (walking on the fingers and toes). The maximum speed of Iguanodon has been estimated at 24 km/h (15 mph), which would have been as a biped; it would not have been able to gallop as a quadruped.

Large three-toed footprints are known in Early Cretaceous rocks of England, particularly Wealden beds on the Isle of Wight, and these trace fossils were originally difficult to interpret. Some authors associated them with dinosaurs early on. In 1846, E. Tagert went so far as to assign them to an ichnogenus he named Iguanodon, and Samuel Beckles noted in 1854 that they looked like bird tracks, but might have come from dinosaurs. The identity of the trackmakers was greatly clarified upon the discovery in 1857 of the hind leg of a young Iguanodon, with distinctly three-toed feet, showing that such dinosaurs could have made the tracks. Despite the lack of direct evidence, these tracks are often attributed to Iguanodon. A trackway in England shows what may be an Iguanodon moving on all fours, but the foot prints are poor, making a direct connection difficult. Tracks assigned to the ichnogenus Iguanodon are known from locations including places in Europe where the body fossil Iguanodon is known, to Spitsbergen, Svalbard, Norway.

The thumb spike is one of the best-known features of Iguanodon. Although it was originally placed on the animal's nose by Mantell, the complete Bernissart specimens allowed Dollo to place it correctly on the hand, as a modified thumb. (This would not be the last time a dinosaur's modified thumb claw would be misinterpreted; Noasaurus, Baryonyx, and Megaraptor are examples since the 1980s where an enlarged thumb claw was first put on the foot, as in dromaeosaurids.)

This thumb is typically interpreted as a close-quarter stiletto-like weapon against predators, although it could also have been used to break into seeds and fruits, or against other Iguanodon. One author has suggested that the spike was attached to a venom gland, but this has not been accepted, as the spike was not hollow, nor were there any grooves on the spike for conducting venom.

Although sometimes interpreted as the result of a single catastrophe, the Bernissart finds instead are now interpreted as recording multiple events. According to this interpretation, at least three occasions of mortality are recorded, and though numerous individuals would have died in a geologically short time span (?10–100 years), this does not necessarily mean these Iguanodon were herding animals.

An argument against herding is that juvenile remains are very uncommon at this site, unlike modern cases with herd mortality. They more likely were the periodic victims of flash floods whose carcasses accumulated in a lake or marshy setting. The Nehden find, however, with its greater span of individual ages, more even mix of Dollodon or Mantellisaurus to Iguanodon bernissartensis, and confined geographic nature, may record mortality of herding animals migrating through rivers.

There is no evidence that Iguanodon was sexually dimorphic (with one sex appreciably different from the other). At one time, it was suggested that the Bernissart I. "mantelli", or I. atherfieldensis (Dollodon and Mantellisaurus, respectively) represented a sex, possibly female, of the larger and more robust, possibly male, I. bernissartensis. However, this is not supported today. A 2017 analysis showed that I. bernissartensis does exhibit a large level of individual variation in both its limbs (scapula, humerus, thumb claw, ilium, ischium, femur, tibia) and spinal column (axis, sacrum, tail vertebrae). Additionally, this analysis found that individuals of I. bernissartensis generally seemed to fall into two categories based on whether their tail vertebrae bore a furrow on the bottom, and whether their thumb claws were large or small.

Evidence of a fractured hip bone was found in a specimen of Iguanodon, which had an injury to its ischium. Two other individuals were observed with signs of osteoarthritis as evidenced by bone overgrowths in their anklebones which are called osteophytes.

Since its description in 1825, Iguanodon has been a feature of worldwide popular culture. Two lifesize reconstructions of Mantellodon (considered Iguanodon at the time) built at the Crystal Palace in London in 1852 greatly contributed to the popularity of the genus. Their thumb spikes were mistaken for horns, and they were depicted as elephant-like quadrupeds, yet this was the first time an attempt was made at constructing full-size dinosaur models. In 1910 Heinrich Harder portrayed a group of Iguanodon in Tiere der Urwelt, a classic German collecting card game about extinct and prehistoric animals.

Several motion pictures have featured Iguanodon. In the 2000 Disney animated film Dinosaur, an Iguanodon named Aladar served as the protagonist with three other iguanodonts as other main and minor characters are Neera, Kron and Bruton. A loosely related ride of the same name at Disney's Animal Kingdom is based around bringing an Iguanodon back to the present. Iguanodon is one of the three dinosaur genera that inspired Godzilla; the other two were Tyrannosaurus rex and Stegosaurus. Iguanodon has also made appearances in some of the many The Land Before Time films, as well as episodes of the television series.

Aside from appearances in movies, Iguanodon has also been featured on the television documentary miniseries Walking with Dinosaurs (1999) produced by the BBC (along with then-undescribed Dakotadon lakotaensis) and played a starring role in Sir Arthur Conan Doyle's book The Lost World as well as featuring in the 2015 documentary Dinosaur Britain. It also was present in Bob Bakker's Raptor Red (1995), as a Utahraptor prey item. A main belt asteroid, 1989 CB₃, has been named 9941 Iguanodon in honour of the genus.

Because it is both one of the first dinosaurs described and one of the best-known dinosaurs, Iguanodon has been well-placed as a barometer of changing public and scientific perceptions on dinosaurs. Its reconstructions have gone through three stages: the elephantine quadrupedal horn-snouted reptile satisfied the Victorians, then a bipedal but still fundamentally reptilian animal using its tail to prop itself up dominated the early 20th century, but was slowly overturned during the 1960s by its current, more agile and dynamic representation, able to shift from two legs to all fours.

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• The Bernissart Iguanodons (Iguanodon herd found in Belgium).
• Mantell's Iguanodon tooth in the collection of the Museum of New Zealand Te Papa Tongarewa