Holsbeek (Dutch pronunciation: [ˈɦɔlzbeːk]) is a municipality located in the Belgian province of Flemish Brabant. The municipality comprises the towns of Holsbeek proper, Kortrijk-Dutsel [nl], Nieuwrode [nl] and Sint-Pieters-Rode [nl]. On January 1, 2006, Holsbeek had a total population of 9,205. The total area is 38.50 km which gives a population density of 239 inhabitants per km.

A notable landmark in Holsbeek is the medieval castle of Horst, which is the fictional home of de rode ridder (the red knight).