Kisoro District is a district in the Western Region of Uganda. The town of Kisoro is the site of the district headquarters.

Kisoro Municipality includes Metropolitan International University, a secular, private educational institution.

Kisoro District is bordered by Kanungu District to the north, Kabale District to the east, Rwanda to the south, and the Democratic Republic of the Congo to the west. The town of Kisoro is approximately 45 kilometres (28 mi), by road, west of Kabale, the largest town in the sub-region.

In 1991, the national population census estimated the district population at 186,870. The population was estimated at 220,300 during the 2002 national census. In 2012, the population was estimated at 254,300. The national population census on 27 August 2014, enumerated the district's population at 281,705 people.

As of September 2002, the religious affiliations of the district residents broke down as follows: Christianity - 95.8 percent, Islam - 0.8 percent, Other - 1.3 percent, None - 2.2 percent.

• Bwindi Impenetrable Forest National Park, one of only four national parks in the world where the African mountain gorilla can be observed in the wild.
• Mgahinga Gorilla National Park has mountain gorillas and covers three of the eight Virunga Volcanoes (Muhabura, Gahinga, and Sabyinyo) all of which lie on the Uganda-Rwanda border.