Kitatsuru (北都留郡, Kitatsuru-gun) is a rural district located in northeastern Yamanashi Prefecture, Japan.

As of October 2020, the district had an estimated population of 1,214 with a density of 7.879 persons per km. The total area was 154.1 km.

Prior to its dissolution, the district consisted of only two villages:

• Kosuge
• Tabayama

Notes

The district formerly included the city of Ōtsuki and a portion of the city of Uenohara.

Kitatsuru District was founded during the early Meiji period establishment of the municipalities system on July 22, 1878 and initially consisted of 18 villages.

Uenohara was elevated to town status on December 27, 1897, followed by Ōtsuki on April 1, 1933. Saruhashi became a town on April 1, 1935, followed by Shippo on April 1, 1954. However, on August 8, 1954, Shippo and Saruhashi merged with Ōtsuki to form the city of Ōtsuki.

• On February 13, 2005 - The former town of Uenohara absorbed the village of Akiyama (from Minamitsuru District) to form the new city of Uenohara.