The Louisville Kentucky Temple is the 76th operating temple of the Church of Jesus Christ of Latter-day Saints (LDS Church). It is physically located in Pewee Valley, Kentucky with a mailing address of Crestwood, Kentucky. The adjacent communities are suburbs of Louisville.

The Louisville Kentucky Temple was announced on March 27, 1999. Thomas S. Monson, of the church's First Presidency, dedicated the Louisville Kentucky Temple on March 19, 2000. The dedication was held after a weeklong public open house.

The Louisville Kentucky Temple has the same design as other small temples built during the same time. The exterior is made of white marble quarried in Vermont and has a single-spire topped with a gold statue of the angel Moroni. It has a total floor area of 10,700 square feet (990 m), two ordinance rooms, and two sealing rooms.

In 2020, the Louisville Kentucky Temple was closed in response to the coronavirus pandemic.