Manitou is an unincorporated urban community in the Municipality of Pembina within the Canadian province of Manitoba that held town status prior to January 1, 2015. The Boundary Trail Railway is based in Manitou. The community's motto is "More Than A Small Town". The community is adjacent to PTH 3 and PR 244. Manitou is located near Mennonite communities such as Winkler, Manitoba and is right next to the St. Leon Wind Farm, the largest wind farm in Manitoba and one of the largest in Canada.

In the 2021 Census of Population conducted by Statistics Canada, Manitou had a population of 812 living in 363 of its 379 total private dwellings, a change of -3.3% from its 2016 population of 840. With a land area of 3.38 km (1.31 sq mi), it had a population density of 240.2/km (622.2/sq mi) in 2021.

Manitou had a weekly newspaper, the Western Canadian which was founded in March 1900 and ran until April 2023 when it was merged with the Pilot Mound Sentinel Courier to form the Hometown View.

Built in 1930, in the Arts and Crafts movement style, the Manitou Opera House is a local heritage landmark known for its unique acoustics. In 2007, Winnipeg folk musician Christine Fellows recorded parts of her album Nevertheless in the Manitou Opera House. It is booked for over 200 events per year.

Two historic houses associated with writer and activist Nellie McClung were renovated and relocated to the town in 2017 and reopened as museums.

• Highway 3
• Provincial Road 244
• Manitou Airport

• Thelma Forbes, politician
• Robert Ironside, businessman
• Nellie McClung, writer

• Media related to Manitou, Manitoba at Wikimedia Commons