Mauchline railway station was a railway station serving the town of Mauchline, East Ayrshire, Scotland. The station was originally part of the Glasgow, Paisley, Kilmarnock and Ayr Railway.

The station opened on 9 August 1848. It was renamed Mauchline for Catrine by 1887, and renamed back to Mauchline in 1903. The station closed to passengers on 6 December 1965.

Today this line is still open as part of the Glasgow South Western Line.

The Garrochburn Goods Depot lay a few miles to the north and until circa 1926 a Mossgiel Tunnel Platform was located just to the north of the tunnel mouth on the Hurlford side.

• Butt, R. V. J. (October 1995). The Directory of Railway Stations: details every public and private passenger station, halt, platform and stopping place, past and present (1st ed.). Sparkford: Patrick Stephens Ltd. ISBN 978-1-85260-508-7. OCLC 60251199. OL 11956311M.
• Jowett, Alan (March 1989). Jowett's Railway Atlas of Great Britain and Ireland: From Pre-Grouping to the Present Day (1st ed.). Sparkford: Patrick Stephens Ltd. ISBN 978-1-85260-086-0. OCLC 22311137.

Preceding station	Historical railways			Following station
Tarbolton Line open; station closed		Glasgow and South Western Railway Ayr to Mauchline Branch		Connection with GPK&AR at Mauchline Junction
Auchinleck		Glasgow and South Western Railway Glasgow, Paisley, Kilmarnock and Ayr Railway		Hurlford Line open; station closed
Catrine Line and station closed		Glasgow and South Western Railway Catrine Branch		Connection with GPK&AR at Brownhill Junction

• Video and commentary on Mossgiel Tunnel, Air Shaft and Tunnel Cottage.

55°30′32″N 4°22′53″W / 55.5090°N 4.3814°W / 55.5090; -4.3814