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Mikesville is an unincorporated community in Columbia County, Florida, United States. It is located along US Routes 41 and 441 with a short overlap of Columbia County Road 18 just north of O'Leno State Park.

Notes

v t e Municipalities and communities of Columbia County, Florida, United States
County seat: Lake City
City	Lake City
Town	Fort White
CDPs	Five Points Watertown
Unincorporated communities	Ellisville Lulu Mikesville
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