Route 110 is a short highway in Jefferson County. Its eastern terminus is at U.S. Route 67 about six miles (10 km) east of De Soto; its western terminus is at Route 21 a few miles north of De Soto. De Soto and Olympian Village are the only cities on the route.

Route 110 begins at an intersection with Route 21 north of De Soto, heading southeast on a two-lane undivided road. The route passes through wooded areas with some fields and homes, crossing over Union Pacific's De Soto Subdivision and coming to an intersection with Route P. The road leaves the De Soto area and heads east through more rural areas with some development. Farther east, Route 110 comes to its eastern terminus at an interchange with US 67 in Olympian Village, where Route CC heads to the north.

In January 2012, the Missouri Highways and Transportation Commission approved the use of Route 110 to include all of Missouri's part of the Chicago–Kansas City Expressway and to correspond to Illinois Route 110 from Hannibal, Missouri to Chicago. The CKC Route 110 follows I-35 from Claycomo northeast of Kansas City to US 36 in Cameron then follows US 36 (and the short part of I-72) to Hannibal. Despite this, the Jefferson County Route 110 remained unchanged.

The intersection with Route 21 was relocated to the south in July 2018.

The entire route is in Jefferson County.

Location	mi	km	Destinations	Notes
De Soto	0.000	0.000	Route 21
	1.544	2.485	Main Street south to Route E
	1.826	2.939	Route P north
Olympian Village	6.294	10.129	US 67 / Route CC north	Interchange
1.000 mi = 1.609 km; 1.000 km = 0.621 mi