Route 34 is a highway in southeastern Missouri. Its eastern terminus is at the Illinois state line on the Mississippi River at Cape Girardeau where it overlaps Route 74. Its western terminus is at U.S. Route 60 near Van Buren. The road continues into Illinois as Illinois Route 146.

Route 34 is one of the original 1922 state highways. Its original eastern terminus was at Jackson.

Spur Route 34 exists in Jackson.

County	Location	mi	km	Destinations	Notes
Carter	​	0.000	0.000	US 60 / Route 21 south	Western end of Route 21 overlap
Reynolds	​	3.367	5.419	Route 21 north – Ellington	Eastern end of Route 21 overlap
Wayne	​	17.939	28.870	Route 49 south – Leeper, Mill Spring	Western end of Route 49 overlap
	Piedmont	25.787	41.500	Route 49 north – Des Arc	Eastern end of Route 49 overlap
	​	35.492	57.119	Route 143 north
	​	39.301	63.249	US 67 – Fredericktown, Greenville, Poplar Bluff	Interchange
Bollinger	Lutesville	70.821	113.975	Route 51 south	Western end of Route 51 overlap
	Marble Hill	71.321	114.780	Route 51 north – Perryville	Eastern end of Route 51 overlap
Cape Girardeau	​	88.153	141.869	Route 72 – Millersville
1.000 mi = 1.609 km; 1.000 km = 0.621 mi       Concurrency terminus