Route 38 is a highway in southern Missouri. Its eastern terminus is at Route 17 ten miles (16 km) west of Houston; its western terminus is at U.S. Route 65 about ten miles (16 km) south of Buffalo.

Major intersections

County	Location	mi	km	Destinations	Notes
Dallas	Jackson Township	0.000	0.000	US 65 / Route TT – Buffalo, Fair Grove	Superstreet intersection Route F / Route M / Route AA / Route EE / Route E / Route J
Webster	Marshfield	22.938	36.915	I-44 – Springfield, Rolla	Route W / Route OO / Route DD / Route A / Route P / Route JJ / Route C / Route F
Wright	Hartville	50.689	81.576	Route 5 – Mansfield, Grovespring	Route Y / Route E
	Van Buren Township	63.632	102.406	Route 95 – Manes, Mountain Grove	Route MM / Route M / Route FF
Texas	Piney Township	80.561	129.650	Route 17 – Houston, Success
1.000 mi = 1.609 km; 1.000 km = 0.621 mi