Route 86 is a highway in southwest Missouri. The eastern terminus is at U.S. Route 65 just north of Ridgedale. From there, the road crosses the Long Creek arm of Table Rock Lake and continues to Blue Eye west between the Arkansas state line on the south and Table Rock Lake on the north. This section is also in the Mark Twain National Forest, and is recommended as a scenic drive by the Missouri Department of Transportation. From Eagle Rock the road turns north to join with Route 76 at Bates Corner with which it is runs concurrent through Cassville to just east of Rocky Comfort. The road continues north and west towards Neosho, then goes further west before turning north towards Joplin where the road ends at the interchange of Interstate 44 and Route 43. Except for a short section in Neosho (where it overlaps with Business I-49), the road is a two-lane highway for its entire length.

The original highway only ran between Ridgedale and Route 43 (now Route 13) north of Blue Eye. It was later extended both east and west. The eastern terminus moving to Kissee Mills, with the route joined with U.S. Route 65 to modern-day Route 76 where it turned east and followed this road to Kissee Mills. It would later be truncated back to its later eastern terminus.

County	Location	mi	km	Destinations	Notes
Newton	Joplin	0.000	0.000	I-44 / Route 43 south – Springfield, Tulsa I-44 BL east / Route 43 north (Hearnes Boulevard)	exit 6 on I-44
	Neosho	16.394	26.384	I-49 / US 71 – Joplin, Fort Smith I-49 BL begins	Western end of Loop 49 overlap
		17.450	28.083	Route 175 north – Tipton Ford
		19.167	30.846	I-49 BL south	Eastern end of Loop 49 overlap
		19.648	31.620	US 60 Bus. west (Neosho Boulevard)	Western end of US 60 Business overlap
		20.371	32.784	US 60 Bus. east (College Street)	Eastern end of US 60 Business overlap
	Neosho–Granby township line	23.920	38.496	US 60 west / Route 59 south – Neosho	Western end of US 60 / Route 59 overlap
		24.170	38.898	US 60 east / Route 59 north – Diamond	Eastern end of US 60 / Route 59 overlap
Barry	Pioneer Township	40.797	65.656	Route 97 north – Pierce City
	Wheaton–Liberty township line	45.779	73.674	Route 76 west – Anderson	West end of Route 76 overlap
	Cassville	58.991	94.937	Route 37 / Route 112 north / Route 37 Bus. begins – Purdy, Washburn, Seligman	West end of Route 112 / Route 37 Bus. overlap
		60.159	96.817	Route 37 Bus. north (Main Street) – Monett	East end of Route 37 Bus. overlap
		60.358	97.137	Route 248 east
	Flat Creek Township	61.915	99.643	Route 112 south – Seligman, Roaring River State Park	East end of Route 112 overlap
		64.579	103.930	Route 76 east to Route 39	East end of Route 76 overlap
	Roaring River Township	77.996	125.522	Route P to AR 23
	White River Township	81.972	131.921	Route H to AR 143
Stone	Carr Lane	86.946	139.926	Route 39 – Shell Knob, Berryville
	Pine Township	95.833	154.228	Route 13 north – Lampe	Western end of Route 13 overlap
		98.665	158.786	Route 13 south – Blue Eye	Eastern end of Route 13 overlap
Taney	Oliver Township	110.324	177.549	US 65 – Branson, Harrison
1.000 mi = 1.609 km; 1.000 km = 0.621 mi       Concurrency terminus