Mittelholstein ("central Holstein") is an Amt ("collective municipality") in the district of Rendsburg-Eckernförde, in Schleswig-Holstein, Germany. It was formed on 1 January 2012 from the three former Ämter Aukrug, Hanerau-Hademarschen and Hohenwestedt-Land and the municipality Hohenwestedt. The seat of the Amt is in Hohenwestedt.

The Amt Mittelholstein consists of the following municipalities:

Arpsdorf Aukrug Beldorf Bendorf Beringstedt Bornholt Ehndorf Gokels Grauel Hanerau-Hademarschen

Heinkenborstel Hohenwestedt Jahrsdorf Lütjenwestedt Oldenbüttel Meezen Mörel Nienborstel Nindorf Osterstedt

Padenstedt Rade bei Hohenwestedt Remmels Seefeld Steenfeld Tackesdorf Tappendorf Thaden Todenbüttel Wapelfeld

54°06′N 9°39′E / 54.1°N 9.65°E / 54.1; 9.65