First opened in 1977, the crescent-shaped Mount Lofty Botanic Garden is situated on 100 hectares on the eastern slopes of Mount Lofty in the Adelaide Hills east of Adelaide in South Australia. The cooler, wetter location suits plants from temperate climates which are difficult to grow on the Adelaide Plains. Amongst the native Australian flora there are tree ferns, as well as exotic cultivated plants from cool climates including Rhododendron and Magnolia and the National Species Rose Collection. The Mount Lofty Botanic Garden, together with the Adelaide and Wittunga Botanic Gardens, is administered by the Botanic Gardens of South Australia, a State Government statutory authority.

In 1980, it was listed on the now-defunct Register of the National Estate.

• List of Adelaide parks and gardens

Aitken, Richard (2006). Seeds of change : an illustrated history of Adelaide Botanic Garden. Adelaide : Board of the Botanic Gardens and State Herbarium.

• Mt Lofty Botanic Garden
• Friends of the Botanic Gardens of Adelaide Retrieved 12 September 2012
• Botanic Gardens of South Australia Retrieved 29 June 2017.