Nebraska Highway 40 is a highway in central Nebraska. It has a length of 85.73 miles (137.97 km). It has a western terminus at Nebraska Highway 92 in Arnold and an eastern terminus north of Kearney at an intersection with Nebraska Highway 10.

Nebraska Highway 40 begins in Arnold at an intersection with Nebraska Highway 92. It goes south out of Arnold into farmland and at an intersection with Nebraska Highway 47, turns east. It continues east until Nebraska Spur 21B, a spur road into Callaway, then turns southeasterly. At Oconto, Highway 40 meets Nebraska Highway 21. It continues in a southeasterly direction through Eddyville and Sumner and at Miller, meets U.S. Highway 183. It continues southeast through Amherst and Riverdale and ends north of Kearney at an intersection with Nebraska Highway 10. Much of the alignment of Nebraska Highway 40 from Oconto southeastward to its eastern terminus lies parallel to the Wood River.

County	Location	mi	km	Destinations	Notes
Custer	Arnold	0.00	0.00	N-92 (Arnold Avenue)	Western terminus
	​	11.10	17.86	N-47 south
	Callaway	25.65	41.28	S-21B north
	Oconto	38.84	62.51	N-21
Dawson	Eddyville	50.29	80.93	S-24C east
Buffalo	Miller	65.00	104.61	US 183 (Armada Street)
	Glenwood Park	85.72	137.95	N-10	Eastern terminus; roundabout
1.000 mi = 1.609 km; 1.000 km = 0.621 mi

• Nebraska Roads: NE 21-40