Negohot (Hebrew: נגוהות) is an Israeli settlement in the southern Hebron Hills of the West Bank. Located on a hill 700 m (2,300 ft) above sea level to the west of the Hebron area, it is organised as a community settlement and falls under the jurisdiction of Har Hevron Regional Council. In 2022 it had a population of 514.

The nearest Israeli locality is Shekef, a moshav a few kilometres over the Green Line in the Hevel Lakhish area of the Shephelah. The international community considers Israeli settlements in the West Bank illegal under international law, but the Israeli government disputes this.

The village was first established in 1982 as a pioneer Nahal military outpost. In 1998, constraints of the Nahal brigade led to the village changing hands and being turned over hesder soldiers who volunteered for extra service to keep a residential presence on that hilltop. The soldiers renovated the area, built a beit midrash and eventually completed their army service and continued to raise families there.

On 25 September 2003, the night of Rosh Hashana, a Palestinian militant from Islamic Jihad killed two residents, including a 7-month-old baby girl, while the family was eating the festive holiday meal.

Many of the adult residents work outside the village, though some entrepreneurs have begun some local small businesses. The children of Negohot travel to the 'Dvir' elementary school in Otniel. Negohot receives assistance from the Amana settlement organization.

• Village website
• Negohot Archived 2011-07-21 at the Wayback Machine Amana
• Negohot Peace Now