The Papel Misionero Natural Cultural Reserve (Spanish: Reserva natural cultural Papel Misionero) is a natural cultural reserve in the Misiones Province of Argentina.

The Papel Misionero Natural Cultural Reserve is 45 kilometres (28 mi) from the town of El Soberbio, Misiones. It has an area of 10,397 hectares (25,690 acres). It is a protected area with managed resources, administered by the Fundación Selva Misionera. The reserve is in the Alto Paraná Atlantic forests ecoregion. The land is privately owned, and visitors are not allowed. It would be part of the proposed Trinational Biodiversity Corridor, which aims to provide forest connections between conservation units in Brazil, Paraguay and Argentina in the Upper Paraná ecoregion.

The land was acquired by the Papel Misionero company in 1978. The Papel Misionero Natural Cultural Reserve was created in 1994. The objective is to protect a mature forest and a community of Guaraní people. It was formalized by provincial law 3.256 of 1995. In 1996 it was made part of the Yabotí Biosphere Reserve by law 3.375. A bill in 2003 proposed that the site be declared of public utility and subject to purchase or expropriation to create a Natural Cultural Reserve under the full control of the province.

The reserve contains three villages of Mbayá people with a population in 2016 of about 340. In 2011 the owner signed an agreement with the communities to recognise their ownership of 370 hectares (910 acres) of land, which could be cultivated, and for mutual cooperation in conservation. The local people would have access to the reserve to extract material for handicrafts, medicine and subsistence.

The reserve contains mountain forest vegetation in a good state of conservation. 70% of the reserve is covered by native forest. Another 30% is being reforested with pine for commercial use. The reserve has diverse ecosystems including lagoons and areas of tree ferns, and giant trees with heights of 33 metres (108 ft) and diameter of 9.5 metres (31 ft).

37 species of mammals have been identified. Mammals include South American tapir (Tapirus terrestris), white-lipped peccary (Tayassu pecari), water opossum (Chironectes minimus), giant anteater (Myrmecophaga tridactyla), greater naked-tailed armadillo (Cabassous tatouay), ocelot (Leopardus pardalis), jaguar (Panthera onca) and black howler (Alouatta caraya). Birds include black-and-white hawk-eagle (Spizaetus melanoleucus), pheasant cuckoo (Dromococcyx phasianellus), rusty-barred owl (Strix hylophila), glittering-bellied emerald (Chlorostilbon lucidus), thick-billed saltator (Saltator maxillosus) and black-throated grosbeak (Saltator fuliginosus).