The R160 is a class of New Technology Train subway cars built for the New York City Subway's B Division. Entering service between 2006 and 2010, they replaced all R38, R40, and NYCT-operated R44 cars, and most R32 and R42 cars. The R160s are very similar to the earlier R143s and later R179s. The biggest difference between the R160 and R143 is the Flexible Information and Notice Display (FIND) system on the R160s in place of static LED maps on the R143s and all A-Division New Technology fleet.

In total, 1,662 cars comprise the R160 class, which consists of two models, the 1,002 Alstom-built R160A cars and the 660 Kawasaki-built R160B cars. The R160A cars are organized in two different configurations, with 372 cars arranged in four-car sets and 630 cars arranged in five-car sets. All R160B cars are in five-car sets, but are subdivided by which propulsion system they use; the majority use the Alstom ONIX propulsion system also found on the R160A cars, while cars 8843-9102 use Siemens SITRAC propulsion.

Kawasaki had little to no problems in delivering the R160B cars, which entered service on August 17, 2006. Alstom was behind the delivery schedule early on for the R160As, which first ran on October 17, 2006. The Metropolitan Transportation Authority exercised options for both contracts, and by June 2010, all R160 cars were in service. Numerous experimental features were added to the R160s through the 2010s. 64 R160A cars were fitted with communications-based train control (CBTC) equipment installed for service on the Canarsie Line (L train), while the rest of the remaining fleet has CBTC equipment installed for service on the Queens Boulevard Line (E, F, M, and R trains).

There are two versions of the R160: the R160A (built by Alstom, numbered 8313–8712, 9233–9802, & 9943–9974) and the R160B (built by Kawasaki, numbered 8713–9232 & 9803–9942). The two car types differ only in a few ways; both can interoperate with each other. The R160As and the majority of the R160Bs utilize the same traction motors as the R142s, while the rest of the R160Bs utilize traction motors similar to those tested on R143s 8205–8212. Both versions of the R160s are very similar to the R143s and R179s, but the three car types are not interoperable with each other due to electrical incompatibilities between them.

The R160 cars are configured in either four-car sets or five-car sets. 372 R160A cars (8313–8652 & 9943–9974) are configured as four-car sets and maintained at East New York Yard for the BMT Eastern Division. All four-car sets are assigned to the J, M and Z trains, but sets 8313-8376 are able to run on the L alongside the line's fleet of R143s. The remaining 630 R160A cars as well as all R160Bs are configured as five-car sets and maintained at Jamaica Yard for the E, F, G and R trains.

The R160A base order was part of a $961,687,121 contract funded in part by a grant from the Federal Transit Administration. The primary base order of the R160 class consisted of 660 cars, 400 R160As to be built by Alstom, and the remaining 260 R160Bs to be built by Kawasaki. The contract included options for further orders, which, if exercised, would have brought the total business with NYCT to about US$2.4 billion, for 1,700 subway cars, and Kawasaki would have manufactured 40% (680 cars) of the 1,700 cars. The R160 fleet was purchased at an average cost of $2.0 million USD per car.

The R160s are equipped with regenerative braking, which allows the cars to capture the braking energy as trains enter a station and transfer it to trains on nearby tracks.

One of the major changes and highlights of the new cars is the addition of an electronic "FIND" (Flexible Information and Notice Display) system, which includes an LCD screen displaying the route, route information, and advertisements, and a tri-color (red, yellow, green) LED strip map which displays the next ten stations, plus five consecutive "further stops" to riders. There are three of these in every car. The display updates the stations at every stop, also giving the number of stops to each station listed. The FIND system replaces a plastic card that had a set route and stations printed on, which was used on the R142, R142A, R143, and R188 subway cars, each of which has 64 amber LED dot station indicators. This allows for instant route or line changes with the correct information, which includes, but is not limited to, the omitting of certain stops.

As part of a 2010 pilot program to increase security and capacity, R160B cars 8713–8722 tested folding seats, CCTV, pivoted grabhandles, and looped stanchions. Following passenger complaints and Kawasaki's refusal to test the same equipment on a set of R142 cars, the program was canceled in 2012 and the equipment was removed. Some of the remaining grabhandles and folding seats from the program have been reused to address a state of emergency in 2017, but have been the subject of vandalism.

In 2016, a non-advertised pilot program saw 20 R160 sets based out of Jamaica Yard testing onboard WiFi. That same year, many cars had their Arts for Transit boards replaced with LCD information screens to display public safety announcements, advertisements, and trivia. These screens are similar to the ones onboard all R143 cars; however, they can display a range of colors instead of just red, orange, and green.

In 2018, R160A car 8395 had its truck suspension system replaced with carbon fiber reinforced plastic "efWING [ja]" leaf springs from Kawasaki, to determine the feasibility of them replacing the heavier coil spring suspension found on all other R160s. R160B car 9116 was also equipped with the new suspension.

In September 2020, a set of R160s received digital display advertising as a pilot test. This is similar to the digital advertising found on R188 cars 7847–7848. Multiple R160s have since had these screens installed in place of the paper advertisements.

The R160 cars began to receive CCTV security cameras in mid-2022 as part of a program to combat the rise of vandalism within the subway system.

The R160 fleet is equipped with communications-based train control (CBTC). 64 R160As (8313–8376) have CBTC equipment installed for use on the Canarsie Line (L train), alongside already-equipped R143 cars. The CBTC equipment used in the Canarsie Line cars was manufactured by Siemens Transportation Systems. R160As 8313–8316 and 8377–8380 were temporarily taken out of service to test equipment between Bergen Street and Church Avenue on the express tracks of the IND Culver Line, and later returned to service.

An additional 1,486 cars, comprising all 5-car sets and many 4-car sets, have been equipped with CBTC for use on the Queens Boulevard Line (E, F, M, and R trains). The CBTC equipment for the Queens Boulevard Line cars is different from the Canarsie Line CBTC equipment and was manufactured by a joint venture between Siemens and Thales Group. As of November 2021, the remaining 4-car sets will be equipped with CBTC for $11.9 million for use on the Eighth Avenue Line.

On July 31, 2002, it was announced that New York City Transit awarded a $961,687,121 contract to Alstom for 660 new cars, with two new options that could provide for a total of 1,040 cars. Kawasaki and Alstom organized a joint venture called Alskaw Inc. for project management, engineering, and equipment purchasing to pursue the contract, and to allow for operational compatibility with the R143s, which were built by Kawasaki. The two companies built and delivered the rolling stock through the joint venture. Kawasaki not only manufactured 260 cars for the base contract but was also the engineering leader for the whole project and provided the trucks for all cars. Alstom assembled 1002 R160A cars at its manufacturing plant in Hornell, New York, while Kawasaki assembled 660 R160B cars at its plant in Yonkers, New York and Kobe, Hyōgo in Japan. Shells for the Alstom-built cars were built in their Lapa plant, in São Paulo, Brazil, and shells for the Kawasaki-built cars were assembled at their Lincoln, Nebraska plant. The base order consisted of 660 cars, the first option included 620 cars, and the second option included 382 cars.

Early on in the order, Alstom encountered significant start-up production problems since being awarded the base contract. In July 2005, Alstom missed its contractual deadline to deliver the 10-car test train, which arrived five months late with Alstom requesting three additional months to deliver the test train. In addition, the Transit Authority rejected several car shells made earlier at their plant in Lapa, Brazil, near São Paulo, after discovering welding defects.