The Roman Catholic Diocese of Amboina (Latin: Amboinaën(sis)) is a diocese located in the city of Amboina in the ecclesiastical province of Makassar in Indonesia.

• It was founded as the Diocese of Amboina in 1534 under Spanish rule and as the seat of St. Francis Xavier.
• As part of the mission of the Diocese of Manila in 1595.
• December 22, 1902: Established as the Apostolic Prefecture of Dutch New Guinea from the Apostolic Vicariate of Batavia
• August 29, 1920: Promoted as the Apostolic Vicariate of Dutch New Guinea
• May 12, 1949: Renamed as Apostolic Vicariate of Amboina
• January 3, 1961: Promoted as Diocese of Amboina

• Bishops of Amboina (Roman rite)
  • Bishop Seno Ngutra (8 December 2021 – present)
  • Bishop Petrus Canisius Mandagi, M.S.C. (10 June 1994 – 11 November 2020)
  • Bishop Andreas Peter Cornelius Sol, M.S.C. (15 January 1965 – 10 June 1994)
  • Bishop Jacques Grent, M.S.C. (3 January 1961 – 15 January 1965)
• Vicars Apostolic of Amboina (Roman Rite)
  • Bishop Jacques Grent, M.S.C. (12 May 1949 – 3 January 1961)
• Vicars Apostolic of Dutch New Guinea (Roman Rite)
  • Bishop Jacques Grent, M.S.C. (10 July 1947 – 12 May 1949)
  • Bishop Giovanni Aerts, M.S.C. (28 August 1920 – 1942. Death execution)
• Prefects Apostolic of Dutch New Guinea (Roman Rite)
  • Fr. Hendrik Nollen, M.S.C. (1915 – 1920)
  • Fr. Matthijs Neyens, M.S.C. (1902 – 1915. Dismissed)

• Steenbrink, Karel (2007), Catholics in Indonesia, 1903-1942 : A Documented History, vol. 2, Brill, ISBN 978-90-67-18260-7

• GCatholic.org
• Catholic Hierarchy

3°42′00″S 128°10′01″E / 3.7000°S 128.1670°E / -3.7000; 128.1670