Route 129 is a highway in northern and central Missouri. Its northern terminus is at the Iowa state line where it continues as Appanoose County Road T20; its southern terminus is at Route 3 in Roanoke.

County	Location	mi	km	Destinations	Notes
Randolph–Howard county line	Roanoke	0.000	0.000	Route 3 – Armstrong, Clifton Hill
Howard	No major junctions
Chariton	Salisbury	12.858	20.693	US 24 west – Keytesville	Southern end of US 24 overlap
		12.966	20.867	US 24 east – Clifton Hill	Northern end of US 24 overlap
Macon	Lingo Township	39.100	62.925	US 36 east / Route 110 (CKC) east – Macon	Southern end of US 36 / Route 110 overlap
Linn	Bucklin Township	44.652	71.860	US 36 west / Route 110 (CKC) west – Brookfield	Northern end of US 36 / Route 110 overlap
	Baker Township	59.446	95.669	Route 11 south – Brookfield	Southern end of Route 11 overlap
	North Salem Township	64.366	103.587	Route 11 north to US 63	Northern end of Route 11 overlap
Sullivan	Green City	83.438	134.280	Route 6 – Milan, Kirksville
Putnam	Richland–Lincoln township line	103.377	166.369	US 136 east – Lancaster	Southern end of US 136 overlap
	Lincoln–Union township line	104.260	167.790	US 136 west – Unionville	Northern end of US 136 overlap
	Lincoln Township	115.300	185.557	CR T20 north – Cincinnati	Continuation into Iowa
1.000 mi = 1.609 km; 1.000 km = 0.621 mi       Concurrency terminus