Route 133 is a highway in central Missouri. Its northern terminus is at U.S. Route 63 near Westphalia; its southern terminus is at Interstate 44 about fourteen miles (21 km) west of Waynesville.

Highway 133 passes through Richland, where the highway intersects with Route 7.

County	Location	mi	km	Destinations	Notes
Pulaski	Gascozark	0.000	0.000	I-44 – Rolla, Springfield	exit 145
Laclede	No major junctions
Pulaski	Richland	6.532	10.512	Route 7 west (Washington Avenue)	Southern end of Route 7 overlap
		7.670	12.344	Route 7 south to I-44	Northern end of Route 7 overlap
	Tavern Township	17.902	28.810	Route 17 south – Waynesville	Southern end of Route 17 overlap
		20.748	33.391	Route 17 north – Iberia	Northern end of Route 17 overlap
Maries	Boone Township	43.504	70.013	Route 42 east – Vienna	Southern end of Route 42 overlap
		43.725	70.369	Route 42 west – Iberia	Northern end of Route 42 overlap
		53.970	86.856	Route 52 west – St. Elizabeth
Osage	Meta	56.214	90.468	Route B – St. Thomas
	Jackson Township	58.684	94.443	Route P – Koeltztown
	Washington Township	71.160	114.521	US 63 – Westphalia, Jefferson City
1.000 mi = 1.609 km; 1.000 km = 0.621 mi       Concurrency terminus