Spider-Girl is the code name of several fictional characters in comic books published by Marvel Comics. The most prominent version and first to receive an ongoing series is Mayday Parker from the MC2 universe, the second version is Anya Corazon, the third version is Gwen Warren, and the fourth version is Makawalu Akana, the latter three from the Earth-616 universe. Several alternate reality incarnations of the character have additionally received notoriety, including the Ultimate Spider-Girl, Ashley Barton, Betty Brant, April, Penelope and Petra Parker, and Charlotte Morales.

The first portrayed Spider-Girl, Mayday Parker, first appeared in a one-shot story in the ongoing series What If. Following positive fan response to the concept, Spider-Girl and two other series (A-Next and J2) set in the same alternate future universe were launched under the MC2 imprint with The Amazing Spider-Girl and Spectacular Spider-Girl. On November 8, 2008, Marvel EIC Joe Quesada confirmed that Spider-Girl would become a feature in the monthly anthology magazine Amazing Spider-Man Family. The series would replace the feature "Mr. and Mrs. Spider-Man", written by DeFalco, which served as a prequel series to the Spider-Girl universe. The title would continue to be simultaneously published in paper form within Amazing Spider-Man Family. Amazing Spider-Man Family #5 (published April 2009) through #8 (July 2009) contained these Spider-Girl stories until the title's cancellation with issue #8, followed by one last Spider-Girl tale, Spider-Girl: The End, in which fellow Spider-Girl April Parker is killed.

In November 2010, a new Spider-Girl series was launched that was unconnected to the MC2 universe. The MC2 Spider-Girl title was cancelled, having surpassed publisher expectations for longevity. The new series featured a new character, Anya Corazon, whose adventures occurred on Earth 616. The series was canceled after only eight issues. No official reason was given for the cancellation. This character returned for a Spider-Island limited series.

The character who would in 2023 become the second Earth-616 Spider-Girl was introduced in Avenging Spider-Man #16 (January 2013), before returning a decade later in X-Men Unlimited Infinity Comic #69 (January 2023) under the name "Gwen Warren", assuming the mantle of Spider-Girl as a member of the X-Men.

The character who would become the third Earth-616 Spider-Girl debuted in Spider-Boy vol. 2 #12 (October 2023), her name of Makawalu Akana, the villainous protégé of Bullseye, revealed the following issue.

The daughter of Peter Parker and Mary Jane Watson from the MC2 universe.

Prior to calling herself "Mayhem", Mayday's clone April goes by Spider-Girl, the two sharing the mantle.

A Latina superhero who originally called herself Araña (Spider), and occasionally goes by Spider-Girl.

The chimeria genetic daughter of Gwen Stacy, Ana Soria, and Scott Summers, grown by the Jackal using DNA stolen from Mr. Sinister, she has the ability to transform into a giant spider with optical lasers. Seeing Scott Summers as her father, she later joins the X-Men Green.

The second volume of Spider-Boy introduces a new incarnation of Spider-Girl who is the supervillain minion of Bullseye. She accompanies him in ambushing Spider-Boy and Daredevil on top of the Daily Bugle to reclaim the trophy. As Spider-Boy is surprised that Spider-Girl knows his true identity and claims that she is like this because of him, Spider-Girl webs up Spider-Boy and Daredevil with her barbed webbing as she and Bullseye get away with the Golden Fang Trophy. This causes Daredevil and Spider-Boy to follow them to Madripoor.

In a flashback to three years ago in Kawainui Falls, Hawaii, Spider-Girl's history as a Hawaiian girl named Makawalu Akana is explored, who after doing a dive off a cliff that was just done by Koa Iona, was approached by Bullseye, who notice her other talents like gymnastics, chess tournaments, and a battle of the bands, led him to take her on as his protégé. In the present, Makawalu and Bullseye are en-route on an unmarked airplane to Madripoor as Bullseye states that they can walk around in their costume in Madripoor. They arrive at the sight of the Challenge of the Jade Dragon overseen by the Gaping Maw and present the Golden Fang Trophy as their entry into the tournament. Bullseye tells Spider-Girl about some of the competitors which consist of Bengal, an Iron Fist user, the Sisters of Stone, the Perfect Golden Duo, and El Gigante and La Mota. Bullseye and Spider-Girl are then surprised that the Challenge of the Jade Dragon got Daredevil and Spider-Boy as the latest competitors.

In "What If Someone Else Besides Spider-Man Had Been Bitten By The Radioactive Spider?", Betty Brant is one of three candidates – along with Flash Thompson and John Jameson – who is bitten by the radioactive spider which gave Spider-Man his powers. After confiding in Peter, and with his assistance, she begins to fight crime under the name "The Amazing Spider-Girl", with a mask similar to Spider-Man's but a very different costume. One time, she fails to stop a certain crook, who subsequently murders Peter's uncle Ben. The shock over the consequences of her failure makes Betty quit her Spider-Girl identity, although Peter takes up the identity of Spider-Man later on by synthetically recreating and ingesting the irradiated spider's venom. This incarnation also appears in the events "Spider-Verse" and "End of the Spider-Verse".

An Ultimate Marvel version of Spider-Girl/Spider-Woman is featured with the Ultimate continuity. This version, known by various names, is a gender-swapped clone of the Peter Parker of the Ultimate Universe, with all of his memories, from their perspective having gone to sleep one day a boy and woken up the next as a girl. Initially known as Spider-Girl/Spider-Woman, she joins the Avengers and takes on the mantle of Black Widow, before returning to their original name.

In Ultimate Spider-Man #200, a glimpse of the future shows Kitty Pryde to eventually become Spider-Girl.

In the pages of Old Man Logan, Ashley is the daughter of Tonya Parker and Hawkeye who did not like the way that Kingpin was running Hammer Falls. She becomes "Spider-Bitch", allying herself with a new Punisher and Daredevil, and plans to take back Hammer Falls, only for the group to be captured and Daredevil and Punisher to be fed to the carnivorous dinosaurs. Hawkeye breaks his daughter out of her cell, whereafter Ashley immediately beheads Kingpin which avenges Daredevil and Punisher's deaths. Then she attempts to kill her father, before taking over Hammer Falls as the new Kingpin. Old Man Logan rescues Hawkeye as Ashley sends her men after them.

The character appears in the "Spider-Verse" and Spider-Geddon storylines, now referred with her father's surname as Ashley Barton, and alternately referred to as "Spider-Girl" and "Spider-Woman" due to the family-friendly nature of the narrative, and is among the spider-powered characters who are recruited by The Superior Spider-Man (Doctor Octopus's mind in Peter Parker's body) to help fight the Inheritors, before returning to the Wastelands in "Venomverse" and "Old Man Quill".

Introduced in "Spider-Verse", Penelope P. Parker is the 11-year-old Spider-Girl of Earth-11, who is best friends with Mary Jane Watson and has a crush on Flash Thompson.

The celebrity daughter of Gwen Stacy and Miles Morales from Earth-8, Charlotte "Charlie" Morales operates as Spider-Girl alongside her brother Max as Spider-Boy.

• Several incarnations of Spider-Girl appear in Spider-Man: Across the Spider-Verse (2023):
  • Mayday Parker, the infant daughter of Peter B. Parker and Mary Jane Parker of Earth-616B.
    • Mayday Parker, an adult with Heterochromia iridum operating as Spider-Girl and a member of Miguel O'Hara's Spider-Society.
    • April Parker, an adult clone of Mayday operating as a blue-suited Spider-Girl and a member of the Spider-Society.
  • Anya Corazon, a short-haired muscular adult operating as a blue-armored Spider-Girl and a member of the Spider-Society.
    • Anya Corazon, a black-and-white-costumed version of Spider-Girl who appears as one of the Spider-People pursuing Miles.
  • Betty Brant, a webbed-suited version of Spider-Girl who appears as one of the Spider-People pursuing Miles.

• An alternate universe version of Spider-Girl named Petra Parker appears in Ultimate Spider-Man, voiced by Olivia Holt.
  • An alternate future version of Petra as Cyborg Spider-Woman appears in Spider-Man: Across the Spider-Verse.
• Anya Corazon / Spider-Girl appears in Spider-Man (2017), voiced by Melanie Minichino. This version is a student at Horizon High. After developing spider-powers during the first season, she later adopts the Spider-Girl alias in subsequent seasons.
• Anya Corazon / Spider-Girl appears in Marvel Super Hero Adventures, voiced by Gigi Saul Guerrero.

An alternate universe variant of May Parker/Spider-Girl originating from Iron Man 2020's timeline appears in the novel Time's Arrow 3: The Future by Tom DeFalco and Rosemary Edghill.

• Spider-Girl appears as an alternate costume for Spider-Woman in Marvel: Ultimate Alliance.
• Spider-Girl makes a non-speaking cameo appearance in Spider-Man: Shattered Dimensions.
• Spider-Girl was a playable character in Marvel Super Hero Squad Online.
• The Mayday Parker and Anya Corazon incarnations of Spider-Girl appear in Spider-Man Unlimited, with the former voiced by Laura Bailey.
• Spider-Girl appears as a playable character in Marvel: Avengers Alliance.
• Ultimate Spider-Woman, referred to as "Spider-Girl", appears as a playable character in Lego Marvel's Avengers via the Spider-Man DLC pack.

Peter Parker #1–4 (March–June 2010) features a Spider-Man advocacy group known as the Spider-Girls in "The Private Life of Peter Parker", consisting of Becky, Emma Paley, and Leila Goldberg, who operate a community service centre in Spider-Man's honour, opposed in merchandising by Teri Hillman, also calling herself Spider-Girl, with the four coming to peace and ultimately all being known as Spider-Girl by the storyline's end.

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