St. Matthew's Episcopal Church and Churchyard is a historic Episcopal church located on St. Mary's Road, Hillsborough, Orange County, North Carolina. The first three bays of the Gothic Revival-style brick church were built between 1825 and 1826, and its rear was extended by another bay in 1868. It features a square entrance tower built in 1830, which was rebuilt in 1850. The tower has a pyramidal spire and lancet windows.

The property was listed on the National Register of Historic Places in 1971. It is located in the Hillsborough Historic District.