State Route 16 (SR 16) is an east–west highway running from Columbus to Coshocton. Its western terminus is at Civic Center Drive (formerly U.S. Route 33) in Downtown Columbus, and its eastern terminus is at US 36. For much of its run through Licking County, and its entire run through Franklin County, State Route 16 follows the path of Columbus' Broad Street. West of Drexel Avenue in Bexley, the route is cosigned with U.S. Route 40 until its endpoint just east of the Scioto River.

The State Route 16 designation was originally applied to the routing carrying U.S. Route 422; when that route was created, SR 16 moved to the routing that had carried State Route 20. SR 16 was rerouted at its east end after the creation of US 36, then later truncated to Coshocton, with the eastern end becoming State Route 416.

County	Location	mi	km	Exit	Destinations	Notes
Franklin	Columbus	0.00	0.00		US 40 west (Broad Street) / Civic Center Drive	Western end of US 40 concurrency
		0.32	0.51		US 23 south / US 33 east / US 62 west / SR 3 south (3rd Street)	Western end of US 62 westbound concurrency
		0.41	0.66		US 23 north / US 33 west / US 62 / SR 3 north (4th Street)	Western end of US 62 eastbound concurrency
		1.08– 1.14	1.74– 1.83		I-71 to I-670 west – Cincinnati, Cleveland	Exit 108B (I-71)
		2.85	4.59		US 62 east (Nelson Road)	Eastern end of US 62 concurrency
	Bexley	3.57	5.75		US 40 east (Drexel Avenue)	Eastern end of US 40 concurrency
	Whitehall	6.97	11.22		SR 317 (Hamilton Road)
	Columbus	7.80– 8.31	12.55– 13.37		I-270 – Wheeling, W.Va., Cleveland	Exit 39 (I-270)
Licking	Pataskala	17.73	28.53		SR 310 south (South Main Street) to I-70 – Kirkersville	Western end of SR 310 concurrency
		17.84	28.71		SR 310 north (Hazelton-Etna Road) / Township Road – Johnstown	Eastern end of SR 310 concurrency
	Granville Township	26.56– 26.65	42.74– 42.89		SR 37 west to SR 161 – Johnstown, New Albany, Granville	Interchange, western end of SR 37 concurrency
		26.65	42.89	Western end of freeway
		26.87– 27.23	43.24– 43.82	27	SR 37 east / SR 661 – Granville, Lancaster	Interchange; eastern end of SR 37 concurrency; southern terminus of SR 661
		27.60	44.42		River Road (TR 131)	no access across SR 16 (right-in/right-out)
	Granville–Newark line	29.24– 29.94	47.06– 48.18	29	Thornwood Crossing
	Newark	30.25	48.68	30	Granville Road	Westbound exit only
		30.49– 31.17	49.07– 50.16	—	Country Club Drive / Church Street	Westbound entrance via West Church Street
		31.96– 32.27	51.43– 51.93	—	North 21st Street
		32.50– 33.02	52.30– 53.14	—	SR 79 south – Heath	Western end of SR 79 concurrency
		32.93	53.00	—	North 11th Street	Westbound exit; eastbound entrance
		33.27– 33.63	53.54– 54.12	—	SR 13 (South 4th Street / North Hudson Avenue)
		34.07	54.83	—	North Buena Vista Street	Former eastbound exit and westbound entrance; closed in 2012
		34.39– 34.64	55.35– 55.75	—	SR 79 north (North Cedar Street) – Nellie	Eastern end of SR 79 concurrency
		35.24– 35.55	56.71– 57.21	—	O'Bannon Avenue
	​	36.15– 39.45	58.18– 63.49	At-grade intersections for 3.3 miles (5.3 km)
	Hanover Township	43.12– 43.42	69.39– 69.88		SR 146 east / CR 273 (Toboso Road) – Hanover, Zanesville	Western terminus of SR 146
Muskingum	Jackson Township	46.40– 46.94	74.67– 75.54	47	SR 586 – Mount Vernon, Frazeysburg, Zanesville
		51.15– 52.09	82.32– 83.83	52	Old Riley Road – Frazeysburg
	Cass Township	55.46– 56.20	89.25– 90.45	56	SR 60 – Dresden, Zanesville, Warsaw
		57.08	91.86	Eastern end of freeway
Coshocton	Jackson Township	67.16	108.08		SR 83 south – New Concord	Western end of SR 83 concurrency
	Coshocton	69.81– 70.06	112.35– 112.75	70	SR 541 (West Chestnut Street) – Roscoe Village, Coshocton
	Tuscarawas Township	70.46	113.39		US 36 / SR 83 north to I-77 – Warsaw, Mount Vernon, Canal Lewisville	Eastern end of SR 83 concurrency
1.000 mi = 1.609 km; 1.000 km = 0.621 mi       Concurrency terminus       Incomplete access