Strathfieldsaye is a suburb of the Greater City of Bendigo in Victoria, Australia. It is located 9 kilometres (5.6 mi) south-east of the Bendigo CBD.

A Strathfieldsaye post office opened on 1 January 1864 and a Strathfieldsaye North office on 8 December 1876. In 1877 Strathfieldsaye was renamed Emu Creek, and Strathfieldsaye North became Strathfieldsaye A railway connected Strathfieldsaye with Bendigo between 1888 and 1958.

In the 2016 census, Strathfieldsaye had a population of 5,428. Strathfieldsaye is one of Bendigo's outer-lying suburbs. Strathfieldsaye and its neighbouring suburbs are among some of the wealthier in Bendigo, but also more expensive to live.

Strathfieldsaye is surrounded by the Bendigo suburbs of Strathdale, Kennington, Spring Gully, Flora Hill and Junortoun, and the outer-rural suburbs of Mandurang, Emu Creek, Axe Creek, Longlea and Eppalock.

2006 saw the commencement of a new sporting complex built in Warne Court, Strathfieldsaye which consists of netball courts, football ovals, cricket nets, cricket pitches and a functions room. This sporting complex is now the home ground of the Strathfieldsaye Storm an Australian rules football and Netball team that plays within the Bendigo Football League and the Strathfieldsaye Cricket Club competing in the Bendigo and District Cricket Association.

Strathfieldsaye Recreation Reserve in Club Court, Strathfieldsaye is home to the following local sporting clubs:

• Strathfieldsaye is represented in football (soccer) by the Strathfieldsaye Colts United Football Club;
• Strathfieldsaye Bowls Club;
• Strathfieldsaye Tennis Club; and
• Sedgewick Cricket Club.
• Strathfieldsaye Dodgers Baseball club

Strathfieldsaye includes two primary schools: St. Francis of the Fields and Strathfieldsaye Primary School. There is also a Catholic Secondary school in nearby Junortoun, Catherine McAuley College (formally Catholic College Bendigo). Strathfieldsaye also includes four kindergartens: Goodstart Early Learning, Assisi Kindergarten, Jenny's Early Learning Centre and Brady Bunch Early Learning Centre (Opening November 2019)

The Strathfieldsaye Shopping Hub on Strathfieldsaye Road is the main commercial activity centre in Strathfieldsaye, comprising a small-scale traditional shopping strip, with an IGA supermarket, post office, bakery, butcher, food take-away, pharmacy, hairdresser salon and vet clinic. There is also a petrol station, car wash, mechanic and pub in this area.