Toolern Vale is a town on the north-western outskirts of Greater Melbourne in Victoria, Australia, 40 km (25 mi) north-west of Melbourne's Central Business District, located within the City of Melton and the Shire of Macedon Ranges local government areas. Toolern Vale recorded a population of 818 at the 2021 census.

Toolern Vale contains a primary school, a CFA fire station, a war memorial and reserve with tennis courts, as well as a community hall often used for antique and collectables auctions.

Toolern Vale is renowned for the numerous horse studs, stables and training facilities which abound. Because of this, the area is often referred to as "Thoroughbred Country". VicRoads has issued special, gold and brown "Thoroughbred Country" licence plates which are often seen being spotted on vehicles from Toolern Vale.

The Post Office opened on 1 January 1871 and was known as Toolern until 1904.

Melton Airfield is a small grass strip airfield located at the intersection of Coburns Road and Diggers Rest - Coimadai Road.

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  Park and playground
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  The Toolern Vale General Store
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  Melton Airfield

Media related to Toolern Vale at Wikimedia Commons

• Emirates Park Stud
• Plane Spotting in Victoria