Pueblo Union Depot is the historic railroad station in Pueblo, Colorado. It was built in the Richardsonian Romanesque style in 1889–1890 and added to the National Register of Historic Places in 1975. It is located within the Union Avenue Historic Commercial District.

Initially the station was served by the Denver & Rio Grande Western Railroad, the Atchison, Topeka and Santa Fe Railway, the Colorado & Southern Railway (which was acquired by the Chicago, Burlington and Quincy Railroad in 1908), the Missouri Pacific Railroad, and the Chicago Rock Island & Pacific Railroad. Today the Union Pacific Railroad and the BNSF Railroad share use of the tracks, and the depot is privately owned. Regular passenger train service no longer exists, though there are proposals such as Front Range Passenger Rail, which would provide service to Denver and Colorado Springs. In addition, the depot has been proposed to operate on Amtrak's Southwest Chief.

Presidents Theodore Roosevelt and Woodrow Wilson arrived at the depot, as did vice-presidential candidate Joe Biden and presidential candidate John Kerry.

Media related to Pueblo Union Depot at Wikimedia Commons

• Official site
• Colorado Historical Society

Preceding station	Atchison, Topeka and Santa Fe Railway			Following station
Bragdon toward Denver		Denver Branch		Baxter toward La Junta
Preceding station	Burlington Route			Following station
Bragdon toward Denver		Denver – Teague Colorado and Southern Railway		Furman toward Teague
Preceding station	Denver and Rio Grande Western Railroad			Following station
Swallows toward Ogden		Royal Gorge Route		Eden toward Denver
Preceding station	Missouri Pacific Railroad			Following station
Terminus		Pueblo – Kansas City		Baxter toward Kansas City