State Trunk Highway 127 (often called Highway 127, STH-127 or WIS 127) is a 14.16-mile (22.79 km) state highway in the U.S. state of Wisconsin. It runs from WIS 16 near Wisconsin Dells east to Interstate 39 (I-39) and WIS 16 in Portage; the highway is located entirely within Columbia County. WIS 127 is maintained by the Wisconsin Department of Transportation (WisDOT).

WIS 127 begins at a junction with WIS 16 and County Trunk Highway WD (CTH-WD) in the Town of Newport, southwest of Wisconsin Dells. From here, the highway heads east through farmland, crossing CTH-O. It enters the Town of Lewiston, passing to the south of Lake Corning. Past the lake, the route meets CTH-XX and CTH-AA in succession before turning southeast; the latter route connects WIS 127 to Briggsville. WIS 127 crosses a creek before turning east to pass Lewiston Elementary School. After crossing a second creek, the route again heads southeast toward Portage. The highway curves south to pass Columbia Correctional Institution, a maximum-security state prison, before entering Portage. In Portage, WIS 127 passes several businesses before terminating at a junction with WIS 16 and exit 89B on I-39.

WIS 127 is located on a former alignment of US 16 (now WIS 16). It was removed from US 16 when the portion of the route from Wisconsin Dells to Portage was realigned on a more direct routing in 1948, bringing it closer to the Wisconsin River. The route then became portions of CTH-WD and CTH-AA before re-entering the Wisconsin State Trunk Highway System in 1956, when WIS 127 was established.

The entire route is in Columbia County.

Location	mi	km	Destinations	Notes
Town of Newport	0.0	0.0	WIS 16	Western terminus
Portage	14.1	22.7	I-39 / WIS 16	Eastern terminus
1.000 mi = 1.609 km; 1.000 km = 0.621 mi

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