Busia District is a district in the Eastern region of Uganda.

Busia District borders Tororo District to the north, Busia County, Kenya to the east, Namayingo District to the south, and to the south-west, and Bugiri District to the west. Busia, Uganda, the site of the district headquarters, is approximately 35 km (22 mi) by road south of Tororo, the nearest large town.

At the 1991 national population census, the population in the district was estimated at 163,600. In 2002, the national census estimated the population at 225,000. In 2012, the mid-year population was estimated at 297,600.

Notable people from Busia District include:

• Aggrey Awori -,Former Uganda Minister of Information Technology (2009-2011)
• Barbara Nekesa Oundo, former state minister for Karamoja affairs and the Busia District women's representative in the parliament (since 2011)
• Benjamin Joseph Odoki, former chief justice of Uganda
• James Munange Ogoola, former principal judge of Uganda
• Denis Onyango, Ugandan international association football goalkeeper
• Gabriel Opio, former minister of gender, culture and labour (2009-2011)
• Fred Wabwire-Mangen, professor of epidemiology, Makerere University School of Public Health
• Erasmus Desiderius Wandera, Roman Catholic bishop, Diocese of Soroti (1980-2007)
• Kevinah Taaka Wanaha Wandera, former Member of Parliament, Busia Municipality (2011-2016)
• Barnabas Nawangwe, Professor of Architecture and current Vice Chancellor of Makerere University

The following points of interest are in the district:

• Majanji landing site on Lake Victoria for fish, which is one of the main foods in the district.
• Busitema Forest is roughly 25 km (10 sq mi); it is bisected by the main highway between Uganda and Kenya in Busia.
• Busitema University is a Government University located within the district.

• Busia County
• Districts of Uganda