The Mykolaiv railway station (Ukrainian: Станція Миколаїв) is a railway station in the city of Mykolaiv. The station first opened in 1908, and is a part of Odesa Railways, within the -state-owned Ukrainian Railways. The station is a major passenger train station, and has direct services to most major cities throughout Ukraine, including Kyiv, Odesa, Kharkiv, Dnipro, and Lviv.

According to the "Investment Atlas of Ukraine", Mykolaiv's railway station receives an average of 1.2 million passengers every year.

The railway station was first opened in 1908, and was originally named Vodopyi (Ukrainian: Водопій). The name then changed to Mykolaiv-Sortuvanny in 1966 before renaming in 1987 to just Mykolaiv.

Many long-distance trains stop at Mykolaiv station. The largest destinations include: Kyiv, Odesa, Kharkiv, Dnipro, and Lviv, Zaporizhzhia, Kryvyi Rih, Kherson, and Ivano-Frankivsk.

During summer and winter holidays, additional trains to Kyiv and Lviv are scheduled to accommodate for holiday travel.

On July 5, 2016, a new railway track running between Kyiv and Kherson opened, which includes a stop in Mykolaiv. The travel time between Kyiv and Mykolaiv is estimated to be about 6 hours.

On November 14, 2022, the first train left Mykolaiv station since the Russian invasion of Ukraine, and headed for its destination of Kyiv.

There are also trains that serve secondary cities, such as to Mykolaiv (Lviv Oblast), Dolynska, Kakhovka, Tokarivka, and Apostolove.