Novotroitske Raion (Ukrainian: Новотроїцький район) was one of the 18 administrative raions (districts) of Kherson Oblast in southern Ukraine. Its administrative center was located in the urban-type settlement of Novotroitske. The raion was abolished on 18 July 2020 as part of the administrative reform of Ukraine, which reduced the number of raions of Kherson Oblast to five. The area of Novotroitske Raion was merged into Henichesk Raion. The last estimate of the raion population was 34,747 (2020 est.)

At the time of disestablishment, the raion consisted of one hromada, Novotroitske settlement hromada with the administration in Novotroitske.